- 9E2 (See other available formats)
- Regulatory Status
- Other Names
- NKp46, NCR1
- Mouse IgG1, κ
- Barcode Sequence
- Ave. Rating
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- Product Citations
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CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is directly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- NKp46-Fc fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Clone 9E2 has been shown to block NK activation through NKp46.6
- Additional Product Notes
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes.
- Application References
AB_2936626 (BioLegend Cat. No. 331955)
- Type I membrane glycoprotein (46 kD)
Expressed on resting and activated NK cells
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.
- Gene ID
- 9437 View all products for this Gene ID
- View information about CD335 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD335 Reagents Request Custom Conjugation
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD335 (NKp46)
Biotin anti-human CD335 (NKp46)
PE anti-human CD335 (NKp46)
Alexa Fluor® 647 anti-human CD335 (NKp46)
Pacific Blue™ anti-human CD335 (NKp46)
Brilliant Violet 421™ anti-human CD335 (NKp46)
PerCP/Cyanine5.5 anti-human CD335 (NKp46)
APC anti-human CD335 (NKp46)
PE/Cyanine7 anti-human CD335 (NKp46)
FITC anti-human CD335 (NKp46)
Brilliant Violet 510™ anti-human CD335 (NKp46)
Brilliant Violet 605™ anti-human CD335 (NKp46)
Brilliant Violet 650™ anti-human CD335 (NKp46)
PE/Dazzle™ 594 anti-human CD335 (NKp46)
Alexa Fluor® 700 anti-human CD335 (NKp46)
Brilliant Violet 711™ anti-human CD335 (NKp46)
APC/Fire™ 750 anti-human CD335 (NKp46)
Alexa Fluor® 488 anti-human CD335 (NKp46)
TotalSeq™-B0101 anti-human CD335 (NKp46)
TotalSeq™-C0101 anti-human CD335 (NKp46)
TotalSeq™-A0101 anti-human CD335 (NKp46)
Brilliant Violet 785™ anti-human CD335 (NKp46)
Ultra-LEAF™ Purified anti-human CD335 (NKp46)
APC/Cyanine7 anti-human CD335 (NKp46) Antibody
PE/Cyanine5 anti-human CD335 (NKp46)
PE/Fire™ 810 anti-human CD335 (NKp46)
TotalSeq™-D0101 anti-human CD335 (NKp46)