Recombinant Human IL-15 (carrier-free)

Pricing & Availability
Other Names
IL-15, MGC9721
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Product Citations
publications
IL-15_Human_Recombinant_BA_090214
MO7e cell proliferation induced by human IL-15.
  • IL-15_Human_Recombinant_BA_090214
    MO7e cell proliferation induced by human IL-15.
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Cat # Size Price Quantity Avail. Save
570302 10 µg 156€
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570304 25 µg 316€
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570306 100 µg 880€
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570308 500 µg 1 680€
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Description

IL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). Interleukin-15 (IL-15) is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes (2). IL-15 plays an important role in immune response and shares many functions with IL-2, for example, stimulating the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8). IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation (9).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human IL-15, amino acids Asn49-Ser162 (Accession# NM_000585), was expressed in E. coli.
Molecular Mass
The 114 amino acid recombinant protein has a predicted molecular mass of 12,773 Da. The DTT-reduced protein migrates at approximately 9kDa and the non-reduced protein migrates at approximately 7kDa by SDS-PAGE. The N-terminal amino acid is Asn.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 2 - 10 ng/ml, corresponding to a specific activity of 0.1 - 0. 5 x 106 units/mg, as determined by the dose dependent stimulation of MO7e cell proliferation.
Application

Bioassay

Recommended Usage

Use when high specific biological activity is required.

Application Notes

This IL-15 protein is biologically active and can be used for in vitro assays.


BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References
  1. Correia DV, et al. 2011. Blood 118:992. (BA) PubMed
Product Citations
  1. Correia DV, et al. 2011. Blood. 118:992. PubMed
  2. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  3. Foltz JA, et al. 2018. Cancers (Basel). 10:. PubMed
Publication Library

Antigen Details

Structure
13 kD single non-glycosylated polypeptide
Distribution

IL-15 is expressed by DC, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes.

Function
IL-15 stimulates the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8).
Bioactivity
stimulatory activities on the proliferation, survival and activation of T lymphocytes and NK cells, and the pathogenesis of rheumatoid arthritis via activating T cells and induces IL-17 production
Cell Sources
adherent peripheral blood mononuclear cells, fibroblasts and epithelial cells
Cell Targets
T lymphocytes, NK cells
Receptors
IL-15Rα, IL-15Rβ and IL-15γc
Cell Type
Hematopoietic stem and progenitors
Biology Area
Immunology, Innate Immunity, Stem Cells, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Grabstein K, et al. Science 264:965-968 1994.
2. Ma A, et al. Annu Rev Immunol 24:657-679 2006.
3. Meresse B, et al. Immunity 21:357-366 2004.
4. Armitage RJ et al, J Immunol 154:483-490 1995.
5. Pulendran B, et al. Eur. J. Immunol. 34:66-73 2004.
6. Bouchard A, et al. J. Leukoc. Biol. 76:162-168 2004.
7. Ratthe C, et al. 2004. J. Leukoc. Biol. 75:893.
8.Feng T, et al. 2008. Cell Immunol 5:189.
9. Rubinstein MP et al, P. Natl. Acad. Sci. USA 103:9166-9171 2006.

Gene ID
3600 View all products for this Gene ID
UniProt
View information about IL-15 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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