Purified Streptavidin

Pricing & Availability
Regulatory Status
Other Names
Streptavidin-PURE, SAV-PU, SAV
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Product Citations
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405150 1 mg 57€
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405151 10 mg 329€
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Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical Data Sheet (pdf)

Product Details

Each vial contains Streptavidin lyophilized in sodium chloride buffer.
Reconstitute the content of the vial in distilled water to the desired concentration.
Storage & Handling
For long-term storage, store the lyophilized streptavidin at -20°C or -70°C. After reconstitution, store the solution between 2°C and 8°C for up to 2 weeks, or store in aliquots at -20°C for up to 6 months.

FC - Quality tested
ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed
  2. Urlaub D, et al. 2020. Curr Protoc Immunol. 131:e111. PubMed
  3. Ridge LA, et al. 2021. Cell Rep. 36:109610. PubMed

Antigen Details

52.8 kD tetrameric protein

Streptavidin binds to biotin with high affinity

Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

Gene ID
View information about Biotin on UniProt.org

Related FAQs

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