Purified Streptavidin

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280302 250 µg 34€
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Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical Data Sheet (pdf)

Product Details

Phosphate-buffered solution containing 0.09% sodium azide.
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.

FC - Quality tested
ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house

Recommended Usage

To coat one 96-well plate dilute 24 µL of Purified Streptavidin into 11.976ml of 1X Coating Buffer (Cat. No. 421701, Coating Buffer 5X concentrate). Final concentration in the coated plate is 2 µg/mL. This recommendation is to perform the protocol for HLA class I ELISA to evaluate Flex-T™ peptide exchange. For other applications the reagent needs to be optimized.

Product Citations
  1. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  2. Wu C, et al. 2020. J Am Chem Soc. 142:12314. PubMed

Antigen Details

52.8 kD tetrameric protein.

Streptavidin binds to biotin with high affinity.

Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

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