- IIB8 (See other available formats)
- Other Names
- Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
- Mouse IgG2a
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The Transcription Factor IIB (TFIIB) is a general factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.Product Details
- Human, Wide mammalian
- Antibody Type
- Host Species
- Recombinant protein
- Phosphate-buffered solution, pH 7.2.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- This antibody should be aliquoted and stored at -20°C. Avoid freeze/thaw cycles.
WB - Quality tested
FA, Gel Supershift, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody recognizes Softag 3 epitope tagged proteins and is a human transcription factor. Clone IIB8 recognizes epitope 52-105 (TQDPSRVG). Nonspecific bands over 250kDa may be observed in some cell lysates.
Additional reported applications (for the relevant formats) include: functional assay, gel supershift, and immunoprecipatation.
- Application References
- Thompson NE and Burgess RR. 1994. Protein Expr. Purif. 5:468.
- Thompson NE, et al. 1995. J. Biol. Chem. 270:4735.
- Duellman SJ, et al. 2004. Protein Expr. Purif. 35:147.
AB_2566803 (BioLegend Cat. No. 668405)
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