- Clone
- IIB8 (See other available formats)
- Other Names
- Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
- Isotype
- Mouse IgG2a
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total lysates (15µg protein) from HeLa (Lane 1), 20nM scrambled siRNA (lane 2), 5nM (Lane 3)and 20nM (Lane 4) TFIIB siRNA treated HeLa cells by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TFIIB antibody, clone IIB8 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder. -
Total lysates (15µg protein) from MCF-7, HeLa, HepG2, Molt-4 and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TFIIB antibody, clone IIB8 (upper). Proteins were visualized using 1:3000 diluted HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M is the MW ladder.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 668405 | 100 µg | 220€ | ||||
The Transcription Factor IIB (TFIIB) is a general factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
Product DetailsProduct Details
- Reactivity
- Human, Wide mammalian
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- This antibody should be aliquoted and stored at -20°C. Avoid freeze/thaw cycles.
- Application
-
WB - Quality tested
FA, Gel Supershift, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody recognizes Softag 3 epitope tagged proteins and is a human transcription factor. Clone IIB8 recognizes epitope 52-105 (TQDPSRVG). Nonspecific bands over 250kDa may be observed in some cell lysates.
Additional reported applications (for the relevant formats) include: functional assay, gel supershift, and immunoprecipatation. - Application References
-
- Thompson NE and Burgess RR. 1994. Protein Expr. Purif. 5:468.
- Thompson NE, et al. 1995. J. Biol. Chem. 270:4735.
- Duellman SJ, et al. 2004. Protein Expr. Purif. 35:147.
- RRID
-
AB_2566803 (BioLegend Cat. No. 668405)
Antigen Details
- Structure
- TFIIB is a 35 kD human transcription factor.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 2959 View all products for this Gene ID
- UniProt
- View information about TFIIB on UniProt.org
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