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Purified anti-Synaptophysin Antibody

Pricing & Availability
Clone
SP17 (See other available formats)
Regulatory Status
RUO
Other Names
SYP, Synaptophysin, Major synaptic vesicle protein P38
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
SP17_Pure_Synaptophysin_Antibody_1_110918_updated
Western blot of purified anti-Synaptophysin antibody (clone SP17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
  • SP17_Pure_Synaptophysin_Antibody_1_110918_updated
    Western blot of purified anti-Synaptophysin antibody (clone SP17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgM. Enhanced chemiluminescence was used as the detection system.
  • SP17_Pure_Synaptophysin_Antibody_2_110918
    ICC staining of purified anti-Synaptophysin antibody (clone SP17) on U251MG cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with 2.5 °g/ml of Alexa Fluor® 647 goat anti-Mouse IgM for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Pure_Synaptophysin_Antibody_3_110918
    IHC staining of purified anti- anti-Synaptophysin antibody (clone SP17) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Pure_Synaptophysin_Antibody_4_110918
    IHC staining of purified anti- anti-Synaptophysin antibody (clone SP17) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • SP17_Pure_Synaptophysin_Antibody_5_070620
    IHC staining of purified anti-Synaptophysin (clone SP17) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. The slide was mounted with Fluoromount-G™, with DAPI. The image was captured with a 40X objective. Scale Bar: 50 µm
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837103 25 µg 81€
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837104 100 µg 221€
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Description

Synaptophysin, also known as major synaptic vesicle protein P38, is encoded by the SYP gene in humans and is located on the short arm of the X chromosome. Synaptophysin is expressed in neuroendocrine cells as well as all neurons in the CNS that participate in synaptic transmission. Synaptophysin is a synaptic vesicle glycoprotein, and interacts with synaptobrevin. It has been implicated in X-linked mental retardation and can be used as a specific marker for cells of the adrenal medulla and pancreatic islets. As such, it can be used to identify tumors that are derived from those cells, including neuroblastoma, retinoblastoma, medulloblastoma, and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a crude synaptic preparation from post mortem human brain.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL for chromogenic staining and 0.5 - 2.0 µg/mL for fluorescent staining is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SP17 reacts with native, denatured and recombinant synaptic vesicle protein synaptophysin expressed in CHO cells. The antibody is particularly valuable for immunocytochemical studies of the brain due to its stability in formalin sections. 

Application References
  1. Frigo DE and McDonnell DP. 2008. Mol. Cancer Ther. 3:659. (WB) PubMed
  2. Wolf AB, et al. 2012. J Alzheimers Dis. 1:217. (ELISA) PubMed
  3. Wolf A, et al. 2012. J Alzheimers Dis. 32:217-232. (ELISA) PubMed
  4. McDonnell D 2008. Mol Cancer Ther. 7:659-669. (WB) PubMed
Product Citations
  1. Yasom S, et al. 2022. FASEB Bioadv. 4:408. PubMed
RRID
AB_2783410 (BioLegend Cat. No. 837103)
AB_2783411 (BioLegend Cat. No. 837104)

Antigen Details

Structure
Synaptophysin is a 313 amino acid protein with an apparent molecular mass of 34 kD.
Distribution

Tissue distribution: Brain, pancreas, adipose and soft tissue, gastrointestinal tract, bone marrow, and immune system.
Cellular Distribution: Plasma and synaptic vesicle membrane.

Function
Synaptophysin plays a role in synaptic vesicle docking and membrane fusion.
Interaction
SRCIN1, also interact with itself to form homohexamer or homotetramer
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Zhang L, et al. 2018. Medicine (Baltimore). 97(29):e11487
  2. Moris, et al. 2018. Anticancer res. 38(2):601
  3. Hami J, et al. 2017. J pediatr Neurosci. 12(3):215
Gene ID
6855 View all products for this Gene ID
UniProt
View information about Synaptophysin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/06/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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  • Purified anti-Synaptophysin
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