Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody

Pricing & Availability
Clone
AM006415 (See other available formats)
Other Names
Spike glycoprotein, S glycoprotein (E2)
Isotype
Human IgG1, κ
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Product Citations
publications
AM006415_PURE_SARS-CoV-2-S-Protein-S1_Recombinant_Antibody_063020.png
Recombinant SARS-CoV-2 S protein S1 (Cat. No. 792904) coated onto Nunc™ Maxisorp™ ELISA plates (Cat. No. 423501) at 2 µg/mL and then incubated with a dilution series of purified anti-SARS-CoV-2 S protein S1 recombinant antibody (clone AM006415). Bound antibodies were detected with biotinylated anti-human IgG secondary antibody followed by avidin-horseradish peroxidase and TMB substrate solution. Absorbance was measured at 450 nm.
  • AM006415_PURE_SARS-CoV-2-S-Protein-S1_Recombinant_Antibody_063020.png
    Recombinant SARS-CoV-2 S protein S1 (Cat. No. 792904) coated onto Nunc™ Maxisorp™ ELISA plates (Cat. No. 423501) at 2 µg/mL and then incubated with a dilution series of purified anti-SARS-CoV-2 S protein S1 recombinant antibody (clone AM006415). Bound antibodies were detected with biotinylated anti-human IgG secondary antibody followed by avidin-horseradish peroxidase and TMB substrate solution. Absorbance was measured at 450 nm.
Cat # Size Price Quantity Avail. Save
938601 25 µg 116€
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938602 100 µg 396€
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Description

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses.   SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell.

This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
SARS-CoV-2
Antibody Type
Recombinant
Host Species
Human
Immunogen
SARS-CoV-2
Formulation
140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA - Quality tested

Recommended Usage

Recommended usage concentration for direct ELISA application is 65 ng/mL.

Application Notes

Note: Clone AM006415 is also referred to as Clone 415-6.

Additional Product Notes

Prior to use, quick spin the antibody vial to ensure recovery of maximum volume.

Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws.

Application References
  1. Wan J, et al. 2020. bioRxiv. doi: https://doi.org/10.1101/2020.05.19.104117
RRID
AB_2876763 (BioLegend Cat. No. 938601)
AB_2876763 (BioLegend Cat. No. 938602)

Antigen Details

Structure
Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
Distribution

Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane

Function
Host cell surface receptor binding
Interaction
ACE2
Ligand/Receptor
ACE2
Biology Area
COVID-19
Antigen References
  1. Walls AC, et al. 2020. Cell. 181(2):281-292.
  2. Yan R, et al. 2020. Science. 367 (6485):1444-1448.
  3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263.
  4. Shang J, et al. 2020. PNAS. 117(21):11727-11734
Gene ID
43740568 View all products for this Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 02/22/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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