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Purified anti-Pax6 Antibody

Pricing & Availability
Clone
AD2.35 (See other available formats)
Regulatory Status
RUO
Other Names
Paired box protein Pax-6, Pax6, Pax, Aniridia type II protein, Oculorhombin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_AD2dot35_Purified_Pax6_Antibody_4_031819.png
ICC staining of purified anti-Pax6 antibody (clone AD2.35) on mouse primary astrocytes. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 1 µg/mL of the primary antibody for 1 hour at room temperature, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 conjugated anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Alexa Fluor® 488 conjugated anti-GFAP antibody (clone SMI 25, Cat. No. 837508) was used to stain astrocytes. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm.
  • 1_AD2dot35_Purified_Pax6_Antibody_4_031819.png
    ICC staining of purified anti-Pax6 antibody (clone AD2.35) on mouse primary astrocytes. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then stained with 1 µg/mL of the primary antibody for 1 hour at room temperature, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 conjugated anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Alexa Fluor® 488 conjugated anti-GFAP antibody (clone SMI 25, Cat. No. 837508) was used to stain astrocytes. Nuclei were counterstained with DAPI. The image was captured with a 60X objective. Scale bar: 50 µm.
  • 2_AD2dot35_Purified_Pax6_Antibody_2_041819_updated
    IHC staining of purified anti-Pax6 antibody (clone AD2.35) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_AD2dot35_Purified_Pax6_Antibody_3_031819.png
    IHC staining of purified anti-Pax6 antibody (clone AD2.35) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_AD2dot35_Purified_Pax6_Antibody_5_080321
    IHC staining of purified anti-Pax6 antibody (clone AD2.35) on frozen mouse cerebellum (A, positive control) and sciatic nerve (B, negative control) tissues. Following fixation (Cat. No. 420801) and permeabilization with 0.5% Triton X-100, the tissue sections were incubated with 5.0 µg/mL of purified anti-Pax6 antibody overnight at 4°C. Alexa Fluor® 594 anti-mouse IgG2a (Cat. No. 407120) antibody was used for 2-hour incubation at room temperature, followed by nuclei counterstain with DAPI (Cat. No. 422801). The slides were then mounted with ProLong™ Gold Antifade Mountant for image acquisition with a 40X objective. Scale bar: 50 μm.
  • 5_AD2dot35_Purified_Pax6_Antibody_1_031819.png
    Western blot of purified anti-Pax6 antibody (clone AD2.35). Lane 1: Molecular weight marker; Lane 2: 10 µg of human cerebellum lysate; Lane 3: 20 µg of mouse brain lysate. Lane4: 30 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence (Cat. No. 436303) was used as the detection system.
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862001 25 µg 85€
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862002 100 µg 221€
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Description

Paired box protein Pax6 is a homeobox transcription factor belonging to a family of 9 proteins, which all share a 128 amino-acid DNA binding domain termed “paired domain” (PD). Homologs of Pax proteins exist in worms, flies, frogs, fish and birds. The Pax family has been shown to play important roles in organogenesis and development. The Pax family of proteins modify transcription activity and protein expression levels primarily through binding to the regulatory region of downstream genes. In addition to the PD domain, some Pax proteins, including Pax6, possess another DNA binding domain, a homeodomain (HD), which preferentially binds to sequence with “TAAT” core motif.

Pax6 was first identified by its homology to the Drosophila Paired gene and is mainly expressed in the central nervous system, eye and pancreas. Mutations of Pax6 result in severe eye development abnormalities. Human heterozygous mutations of Pax6 result in aniridia, blindness, cataract and colobomas. In mice, homozygous Pax6 mutations lead to complete loss of ocular structure. In Drosophila, mutation of Pax6 homolog results in an eyeless phenotype. In addition to the eye development, pancreas abnormalities have been also observed in Pax6 deficiency mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated peptide corresponding to amino acid sequence 174-188 of human Pax6.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested
IHC-P, IHC-F, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For formalin-fixed paraffin-embedded immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For frozen tissue immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity is weaker in Rat compared to Human and Mouse.

Product Citations
  1. Feng H, et al. 2021. Stem Cell Research. 53:102346. PubMed
  2. Cai T, et al. 2021. Cell Reports. 36(2):109337. PubMed
  3. Kano M, et al. 2019. Endocrinology. 160:1701. PubMed
RRID
AB_2801237 (BioLegend Cat. No. 862001)
AB_2801238 (BioLegend Cat. No. 862002)

Antigen Details

Structure
Human Pax6 is a 422 amino acid protein with a molecular mass of 46 kD.
Distribution

Tissue distribution: central nervous system, eye, and pancreas
Cellular distribution: nucleus

Function
Pax6 is involved in the regulation of gene expression.
Interaction
Interacts with MAF and MAFB; interacts with TRIM11 Cellular targets: more than 200 target genes, including foxp2, pax6a, gata3, putf3 and more.
Cell Type
Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Chi N, et al. 2002. Trends Genet. 18(1): 41-7.
  2. Coutinho P, et al. 2011. Genome Res. 21(8):1349-59.
Gene ID
5080 View all products for this Gene ID 18508 View all products for this Gene ID 25509 View all products for this Gene ID
UniProt
View information about Pax6 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Pax6 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Pax6 AD2.35 ICC,IHC-P,IHC-F,WB
Go To Top Version: 2    Revision Date: 05/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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