- 1F11 (See other available formats)
- Other Names
- P2X7 receptor (P2X7R), P2RX7
- Rat IgG2b, κ
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- Product Citations
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P2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.Product Details
- Antibody Type
- Host Species
- Murine colon mast cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, IHC, WB - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting1, immunohistochemistry1, in vivo inhibition of intestinal inflammation and mast cell activation1.
- Application References
- Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)
AB_2564122 (BioLegend Cat. No. 148702)
- Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.
Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.
- Ion channel activated by extracellular ATP.
- ATP, NAD.
- Cell Type
- B cells, Macrophages, Mast cells, T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Antigen References
1. Surprenant A, et al. 1996. Science. 272:735.
2. Chused TM, et al. 1996. J. Immunol. 157:1371.
3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911.
4. Kawamura H, et al. 2006. J. Immunol. 176:2152.
5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908.
6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638.
7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.
- Gene ID
- 18439 View all products for this Gene ID
- View information about P2X7R on UniProt.org
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