Purified anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
1-1A8_Pure_061708.jpg
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 1-1A8_Pure_061708.jpg
    C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 2-1A8_Purified_Ly-6G_Antibody_IHC_102518
    Fresh, frozen mouse spleen was stained with purified Ly6G clone 1A8 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
  • 3-1A8_Purified_Ly-6G_Antibody_IHC_022013
    Mouse uterine tissue fixed in 10% formalin, paraffin embedded, and sliced to 4 µm. After deparaffination and antigen retrieval, sample was stained using an automatic slide stainer. The anti-mouse Ly6G primary antibody was applied at 1:500 dilution in blocking buffer for 1 hr at RT and DAB was used for visualization.
Cat # Size Price Quantity Avail. Save
127601 50 µg 44€
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127602 500 µg 92€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Validated
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Guiducci C, et al. 2010. J Exp Med. 207:2931. PubMed
  2. Sumagin R, et al. 2010. J Immunol. 185:7057. PubMed
  3. Reeves R 2010. J Immunol. 185:4904. PubMed
  4. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  5. Cain D, et al. 2011. PLoS One. 6:e19957. PubMed
  6. Raccosta L, et al. 2013. J Exp Med. 210:1711. PubMed
  7. Andrade E, et al. 2013. J Immunol. 191:4759. PubMed
  8. Ng L, et al. 2011. J Invest Dermatol. 131:2058. PubMed
  9. McCartney-Francis N, et al. 2014. J Leukoc Biol. 96:917. PubMed
  10. Her Z, et al. 2015. EMBO Mol Med. 7:24. PubMed
  11. Ilyas G, et al. 2015. J Hepatol. Available online 29 August 2015. PubMed
  12. Yago T, et al. 2015. J Exp Med. 212: 1267 - 1281. PubMed
  13. Sacramento L, et al. 2015. Infect Immun . 83: 4604 - 4616. PubMed
  14. Stempel H, et al. 2016. J Biol Chem. 291: 9762-9775. PubMed
  15. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  16. Lalazar G, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G377 - G386. PubMed
  17. Danobeitia J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183701. PubMed
  18. Watanabe K, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006513. PubMed
  19. Davies M, et al. 2017. PLoS Pathogens. 13(6):e1006435. PubMed
  20. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  21. Bonnart C, et al. 2017. Parasite Immunol.. 10.1111/pim.12489. PubMed
  22. Maybin JA, et al. 2018. Nat Commun. 9:295. PubMed
  23. Meers GK, et al. 2018. PLoS One. 13:e0190846. PubMed
  24. Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed
  25. Qu S, et al. 2018. Int J Cancer. 8:6685. PubMed
  26. Vallelian F, et al. 2018. Pharmacol Res Perspect. 15:5422. PubMed
  27. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  28. Otxoa–de–Amezaga A, et al. 2019. Acta Neuropathol. 137:321. PubMed
  29. Simon N, et al. 2019. JCI Insight. 4:. PubMed
  30. Perdomo J, et al. 2019. Nat Commun. 10:1322. PubMed
  31. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  32. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  33. Boeckel SRV, et al. 2019. Sci Rep. 9:7356. PubMed
  34. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  35. Bianchi–Frias D, et al. 2019. Mol Cancer Res. 17:321. PubMed
  36. Lin L, et al. 2018. J Invest Dermatol. 138:1032. PubMed
  37. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  38. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  39. Vasamsetti SB et al. 2018. Immunity. 49(1):93-106 . PubMed
  40. Wang B et al. 2018. Cell stem cell. 22(2):206-220 . PubMed
  41. Bence Daniel et al. 2018. Immunity. 49(4):615-626 . PubMed
  42. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  43. Chudnovskiy A et al. 2016. Cell. 167(2):444-456 . PubMed
  44. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  45. Mottahedin A, et al. 2017. J Leukoc Biol. 101:297. PubMed
  46. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  47. Ajona D, et al. 2017. Cancer Discov. 0.773611111. PubMed
  48. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  49. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  50. Goel S, et al. 2017. Nature. 548:471. PubMed
  51. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  52. Verma M, et al. 2018. Brain Behav Immun. 69:223. PubMed
  53. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
  54. Schmid AS, et al. 2018. Rheumatology (Oxford). 57:748. PubMed
  55. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  56. Shen S, et al. 2018. Front Immunol. 1.06875. PubMed
  57. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  58. Gu H, et al. 2018. Aging (Albany NY). 1.525694444. PubMed
  59. Luo J, et al. 2019. Clin Cancer Res. 25:808. PubMed
  60. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  61. Bhatt K, et al. 2018. mSphere. 3:e00352. PubMed
  62. Zhang M, et al. 2018. Front Immunol. 2.285416667. PubMed
  63. Düsedau HP, et al. 2019. Glia. 67:193. PubMed
  64. Ford J, et al. 2019. Front Immunol. 2.502083333. PubMed
  65. Kapellos TS, et al. 2019. FASEB J. 33:6154. PubMed
  66. Otxoa–de–Amezaga A, et al. 2019. Stroke. 50:1548. PubMed
  67. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  68. Carper MB, et al. 2019. Cell Rep. 29:1660. PubMed
  69. Wang Y, et al. 2019. Cell. 179:1144. PubMed
RRID
AB_1089179 (BioLegend Cat. No. 127601)
AB_1089180 (BioLegend Cat. No. 127602)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 10/25/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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