Purified anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
1-1A8_Pure_061708.jpg
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 1-1A8_Pure_061708.jpg
    C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
  • 2-1A8_Purified_Ly-6G_Antibody_IHC_102518
    Fresh, frozen mouse spleen was stained with purified Ly6G clone 1A8 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
  • 3-1A8_Purified_Ly-6G_Antibody_IHC_022013
    Mouse uterine tissue fixed in 10% formalin, paraffin embedded, and sliced to 4 µm. After deparaffination and antigen retrieval, sample was stained using an automatic slide stainer. The anti-mouse Ly6G primary antibody was applied at 1:500 dilution in blocking buffer for 1 hr at RT and DAB was used for visualization.
Cat # Size Price Quantity Avail. Save
127601 50 µg 44€
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127602 500 µg 160€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Validated
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Guiducci C, et al. 2010. J Exp Med. 207:2931. PubMed
  2. Sumagin R, et al. 2010. J Immunol. 185:7057. PubMed
  3. Reeves R 2010. J Immunol. 185:4904. PubMed
  4. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  5. Cain D, et al. 2011. PLoS One. 6:e19957. PubMed
  6. Raccosta L, et al. 2013. J Exp Med. 210:1711. PubMed
  7. Andrade E, et al. 2013. J Immunol. 191:4759. PubMed
  8. Ng L, et al. 2011. J Invest Dermatol. 131:2058. PubMed
  9. McCartney-Francis N, et al. 2014. J Leukoc Biol. 96:917. PubMed
  10. Her Z, et al. 2015. EMBO Mol Med. 7:24. PubMed
  11. Ilyas G, et al. 2015. J Hepatol. Available online 29 August 2015. PubMed
  12. Yago T, et al. 2015. J Exp Med. 212: 1267 - 1281. PubMed
  13. Sacramento L, et al. 2015. Infect Immun . 83: 4604 - 4616. PubMed
  14. Stempel H, et al. 2016. J Biol Chem. 291: 9762-9775. PubMed
  15. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  16. Lalazar G, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G377 - G386. PubMed
  17. Danobeitia J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183701. PubMed
  18. Watanabe K, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006513. PubMed
  19. Davies M, et al. 2017. PLoS Pathogens. 13(6):e1006435. PubMed
  20. Nakamizo S,et al. 2017. Sci Rep. . 10.1038/s41598-017-14292-1. PubMed
  21. Bonnart C, et al. 2017. Parasite Immunol.. 10.1111/pim.12489. PubMed
  22. Maybin JA, et al. 2018. Nat Commun. 9:295. PubMed
  23. Meers GK, et al. 2018. PLoS One. 13:e0190846. PubMed
  24. Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed
  25. Qu S, et al. 2018. Int J Cancer. 8:6685. PubMed
  26. Vallelian F, et al. 2018. Pharmacol Res Perspect. 15:5422. PubMed
  27. Mrdjen D 2018. Immunity. 48:380. PubMed
  28. Vasamsetti SB 2018. Immunity. 49:93. PubMed
  29. Snell LM 2018. Immunity. 49:678. PubMed
  30. Daniel B 2018. Immunity. 49:615. PubMed
  31. An Y, et al. 2018. Cell Rep. 25:702. PubMed
  32. Wang B, et al. 2018. Cell Stem Cell. 22:206. PubMed
  33. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  34. Otxoa–de–Amezaga A, et al. 2019. Acta Neuropathol. 137:321. PubMed
  35. Simon N, et al. 2019. JCI Insight. 4:. PubMed
  36. Perdomo J, et al. 2019. Nat Commun. 10:1322. PubMed
  37. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  38. Burzynski LC, et al. 2019. Immunity. 50:1033. PubMed
  39. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  40. Boeckel SRV, et al. 2019. Sci Rep. 9:7356. PubMed
  41. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  42. Bianchi–Frias D, et al. 2019. Mol Cancer Res. 17:321. PubMed
  43. Lin L, et al. 2018. J Invest Dermatol. 138:1032. PubMed
Publication Library
RRID
AB_1089179 (BioLegend Cat. No. 127601)
AB_1089180 (BioLegend Cat. No. 127602)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 10/25/2018

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