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Purified anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
XMG1.2 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG1, κ
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Product Citations
publications
R4-6A2
  • R4-6A2
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505801 50 µg 67€
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505802 500 µg 109€
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
IHC, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Application References
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
  10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
  14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
  17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
  18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
  19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
  21. Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
Product Citations
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  12. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
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  14. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
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  19. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
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  22. Willimsky G, et al. 2021. Elife. 10:. PubMed
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  24. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
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  29. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
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  32. Kamdar K, et al. 2018. J Immunol. 201:230. PubMed
  33. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  34. Juneja VR, et al. 2017. J Exp Med. 214:895. PubMed
  35. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  36. Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed
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  45. Riggan L, et al. 2020. Cell Rep. 31:107651. PubMed
  46. Tonkin D, et al. 2008. J Immunol. 181:4516. PubMed
  47. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  48. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
  49. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  50. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  51. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  52. Montfort M, et al. 2004. J Immunol. 173:4084. PubMed
  53. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  54. Kim SJ, et al. 2020. Cell Mol Immunol. 17:728. PubMed
  55. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  56. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  57. Ranjan K, et al. 2021. J Clin Invest. 131:. PubMed
  58. Van Dis E, et al. 2022. PLoS Pathog. 18:e1010721. PubMed
  59. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  60. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  61. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  62. Ko S, et al. 2005. J Immunol. 175:3309. PubMed
  63. Xu G, et al. 2007. J Immunol. 179:5358. PubMed
  64. Dudek M, et al. 2021. Nature. 592:444. PubMed
  65. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  66. Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed
  67. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  68. Mittal D, et al. 2017. Cancer Immunol Res. 5:1098. PubMed
  69. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  70. Sun Y, et al. 2021. Nat Commun. 12:5147. PubMed
  71. Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
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  73. Lee J, et al. 2007. Nat Immunol. 8:181. PubMed
  74. Kaushik DK, et al. 2019. J Clin Invest. 130. PubMed
  75. García-Lozano A, et al. 2018. Front Microbiol. 9:1275. PubMed
  76. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
  77. Haring J, et al. 2008. J Immunol. 180:2855. PubMed
  78. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  79. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  80. Lee JK, et al. 2021. Cell Mol Immunol. 18:1395. PubMed
  81. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  82. Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
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RRID
AB_315395 (BioLegend Cat. No. 505801)
AB_315396 (BioLegend Cat. No. 505802)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/16/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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