Purified anti-mouse FOXP3 Antibody

Pricing & Availability
Clone
MF-14 (See other available formats)
Regulatory Status
RUO
Other Names
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Isotype
Rat IgG2b, κ
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Product Citations
publications
MF-14_PURE_FOXP3_WB_061919
Total cell lysate from 293E (lane 1) and 293E transfected with plasmid encoding mouse FOXP3 (lane 2) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL of purified anti-mouse FOXP3 antibody (clone MF-14) (upper) or 1:3000 diluted purified anti-GAPDH antibody (clone Poly6314) (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-rat IgG secondary antibody conjugated to HRP for anti-mouse FOXP3 antibody, and a donkey anti-rabbit IgG antibody conjugated to HRP for anti-GAPDH antibody. Lane M: Molecular weight ladder.
  • MF-14_PURE_FOXP3_WB_061919
    Total cell lysate from 293E (lane 1) and 293E transfected with plasmid encoding mouse FOXP3 (lane 2) (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL of purified anti-mouse FOXP3 antibody (clone MF-14) (upper) or 1:3000 diluted purified anti-GAPDH antibody (clone Poly6314) (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted anti-rat IgG secondary antibody conjugated to HRP for anti-mouse FOXP3 antibody, and a donkey anti-rabbit IgG antibody conjugated to HRP for anti-GAPDH antibody. Lane M: Molecular weight ladder.
Cat # Size Price Quantity Check Availability Save
126401 25 µg 88,00€
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126402 100 µg 180,00€
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Description

FOXP3 is a 47 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 150D monoclonal antibody reacts with human, mouse and rat FOXP3. The 150D antibody recognizes FOXP3 epitope encoded by exon 2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB, ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): 0.01 - 1.0 µg/mL antibody in dilution buffer. Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References
  1. Ono M, et al. 2007. Nature 446:685.
  2. Hori S, et al. 2003. Science 299:1057.
  3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
  4. Fallarino F, et al. 2009. J. Immunol. 183:6033. PubMed
  5. Barber A, et al. 2009 J. Immunol. 183:6939. PubMed
  6. Nakashima H, et al. 2010. J. Immunol. 184:4637. PubMed
Product Citations
  1. Pittala S, et al. 2018. Neoplasia. 9:20222. PubMed
  2. Drashansky TT, et al. 2019. Sci Adv. 5:eaaw0480. PubMed
  3. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  4. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  5. Zhang X, et al. 2020. Int J Endocrinol. 2020:6543593. PubMed
  6. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  7. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  8. Artham S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L750:318. PubMed
RRID
AB_1089120 (BioLegend Cat. No. 126401)
AB_1089119 (BioLegend Cat. No. 126402)

Antigen Details

Structure
50-55 kd protein. Forkhead/winged-helix transcription factor family, contains zinc finger and forkhead domains.
Distribution

Nuclear; expressed in Treg cells.

Function
Master regulatory gene in Treg cell development, crucial for immune homeostasis.
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Ono M, et al. 2007. Nature 446:685.
2. Hori S, et al. 2003. Science 299:1057.
3. Fontenot JD, et al. 2003 Nature Immunol 4:330.
4. Fallarino F, et al. 2009. J. Immunol. 183:6033.
5. Barber A, et al. 2009 J. Immunol. 183:6939.
6. Nakashima H, et al. 2010. J. Immunol. 184:4637.

Regulation
Present at high level in T reg cells. Induced by T cell activation.
Gene ID
20371 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

Related FAQs

Can I stain whole blood with anti-FOXP3 using your Foxp3 staining kit?

It is not recommended. It is best to use PBMCs for this testing.

Can FOXP3 be costained with cytokines?

The larger holes created by the nuclear permeabilization required for FOXP3 may allow cytokines to leak out of the cell, making it harder to detect lowly-expressed cytokines. You may have to use a control where the cells are only permeabilized through the cell membrane.

Go To Top Version: 5    Revision Date: 06/19/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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