Purified anti-mouse CD54 Antibody

Pricing & Availability
Clone
YN1/1.7.4 (See other available formats)
Regulatory Status
RUO
Other Names
ICAM-1, Ly-47
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
YN1_1_7_4_Purified_083107
C57BL/6 mouse splenocytes stained with purifid YN1/1.7.4 , followed by anti-rat IgG FITC.
  • YN1_1_7_4_Purified_083107
    C57BL/6 mouse splenocytes stained with purifid YN1/1.7.4 , followed by anti-rat IgG FITC.
  • YN1_1_7_4_Purified_IHC_012017
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with purified 10 µg/mL of CD54 (clone YN1/1.7.4) over night at 4°C, followed by 2.5 µg/mL of anti-rat IgG Alexa Fluor® 594 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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116101 50 µg 36,00€
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116102 500 µg 92,00€
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Description

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse NS-1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).

Application References
  1. Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
  2. Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
  3. Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
  4. Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
  5. Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
  6. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  7. Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
  8. Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
  9. El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
  10. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Saitoh S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01687-x. PubMed
  2. Wolf D, et al. 2018. Nat Commun. 9:525. PubMed
  3. Echeverria GV, et al. 2018. Nat Commun. 9:5079. PubMed
  4. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  5. Tong CF, et al. 2018. Am J Physiol Cell Physiol. 315:C409. PubMed
  6. Clark VD, et al. 2018. Brain Res. 1699:158. PubMed
  7. Yun S, et al. 2019. J Clin Invest. 130:4863. PubMed
  8. Chen PY, et al. 2019. Nat Metab. 0.675. PubMed
  9. Douglas G, et al. 2019. Cardiovasc Res. :. PubMed
  10. Mentrup T, et al. 2019. J Exp Med. 216:807. PubMed
  11. Matsui Y 2007. Radiology . 243:386. PubMed
  12. Liu J, et al. 2010. Proc Natl Acad Sci U S A. 107:16530. PubMed
  13. Seidler D, et al. 2011. J Immunol. 187:6108. PubMed
  14. El-Assaad F, et al. 2013. Infect Immun . 81:3984. PubMed
  15. Greineder C, et al. 2013. PLoS One. 14:80110. PubMed
  16. Chacko A, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PubMed
  17. Yun S, et al. 2016. Nat Cell Biol. 18: 1043-1053. PubMed
  18. Lorsung RM, et al. 2020. Front Bioeng Biotechnol. 0.9375. PubMed
  19. Reglero-Real N, et al. 2021. Immunity. :. PubMed
  20. Wilson ZS, et al. 2020. Sci Rep. 10:2142. PubMed
RRID
AB_313692 (BioLegend Cat. No. 116101)
AB_313693 (BioLegend Cat. No. 116102)

Antigen Details

Structure
Ig superfamily, 90 kD
Distribution

Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Immune reaction, inflammation, adhesion
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Gene ID
15894 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 12/13/2016

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