Purified anti-mouse CD19 Antibody

Pricing & Availability
Clone
1D3/CD19 (See other available formats)
Other Names
B4, CVID3
Isotype
Rat IgG2a, κ
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Product Citations
publications
1D3slashCD19_PURE_CD19_Antibody_080516
C57BL/6 mouse splenocytes were stained with purified CD19 (clone 1D3/CD19, filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
  • 1D3slashCD19_PURE_CD19_Antibody_080516
    C57BL/6 mouse splenocytes were stained with purified CD19 (clone 1D3/CD19, filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
Cat # Size Price Quantity Avail. Save
152402 500 µg 188€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfected k562 cells expressing the extracellular domain of murine CD19.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.

Application References
  1. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
  2. Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
Product Citations
  1. Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
  2. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  3. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  4. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  5. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  6. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  7. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  8. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  9. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  10. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
RRID
AB_2629714 (BioLegend Cat. No. 152402)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD.
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells.

Function
Modulates B cell activation and differentiation.
Ligand/Receptor
CD21, CD81, Leu-13.
Cell Type
B cells, Dendritic cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/09/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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