- 1502022 (See other available formats)
- Other Names
- Matrix metallopeptidase 8, CLG1, MMP8, PMN-CL, Neutrophil Collagenase, MMP-8
- Mouse IgG2b, κ
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- Product Citations
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MMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β, IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.Product Details
- Antibody Type
- Host Species
- Human MMP-8, amino acids (Phe21-Gly467) (Accession# NP_002415.1), is expressed with a C-terminal His tag and a linker sequence in 293E cell lines.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IHC-P - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
AB_2617008 (BioLegend Cat. No. 688002)
- 467 amino acids, SDS-PAGE apparent molecular weight of Pro-MMP8 is 65-75 kD and Mature MMP8 is 55-60 kD. Consists of a prodomain that is cleaved upon activation, a catalytic domain, a hinge region and a carboxyl terminal domain with hemopexin-like repeats
Neutrophils, macrophages, and monocytes.
- Collagen type I, II, and III, gelatin, proteoglycans, fibronectin, aggrecan, substance P, serpins, ß-casein, and angiotensin.
- Cell Type
- Neutrophils, Macrophages, Monocytes
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Enzymes and Regulators
- Antigen References
1. Nagase H, Woessner JF Jr. 1999. J. Biol. Chem. 274:21491.
2. Gill SE, Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334.
3. Mattey DL, et al. 2012. Arthritis Res. Ther. 14:R204.
4. Fang C, et al. 2013. Cardiovasc. Res. 99:146.
5. Lempinen M, et al. 2013. Ann. Med. 45:482.
6. Sommer K, et al. 2013. PLoS One 8:e73992.
- Gene ID
- 4317 View all products for this Gene ID
- View information about MMP-8 on UniProt.org
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