Purified anti-human STAT3 Antibody

Pricing & Availability
Clone
15H2B45 (See other available formats)
Other Names
Signal transducer and activator of transcription 3, acute-phase response factor (APRF), HIES, ADMIO
Isotype
Mouse IgG1, κ
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Product Citations
publications
15H2B45_PURE_STAT3_Antibody_082216
HeLa cells (filled histogram) or PC-3 cells (open histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with purified STAT3 (clone 15H2B45), followed by anti-mouse IgG PE.
  • 15H2B45_PURE_STAT3_Antibody_082216
    HeLa cells (filled histogram) or PC-3 cells (open histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with purified STAT3 (clone 15H2B45), followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
371802 100 µg 180€
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Description

STAT3 is an 88 kD member of the STAT (signal transducer and activators of transcription) protein family that is phosphorylated in response to cytokine receptor-associated kinase activity. Phosphorylation of STAT3 induces nuclear translocation to activate transcription. STAT3 forms both homo- and heterotrimers and is involved in the activation of genes required for cell growth and apoptosis. STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by Rac1 protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human STAT3 recombinant protein (621-770 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 15H2B45 blocks clone 4G4B45.

RRID
AB_2629732 (BioLegend Cat. No. 371802)

Antigen Details

Structure
770 amino acids with a predicted molecular weight of 88.1 kD. Contains a C-terminal SH2 domain responsible for homo- or heterodimerization.
Distribution
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to nucleus to act as transcriptional activator. Activates a wide variety of genes involved in cell growth and apoptosis, involved in gp130-related signaling.
Function
Cytoplasm.
Interaction
STAT3 forms a homodimer or a heterodimer with other STAT family members. Interacts with IL31RA, NCOA1, PELP1, SIPAR, SOCS7, STATIP1, CAV2, DAPK3, EIF2AK2, PIAS3, and TMF1.
Cell Type
Mesenchymal Stem Cells, Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lufei C, et al. 2003. EMBO J. 22:1325.
2. Deo D, et al. 2002. J. Biol. Chem. 277:21237.
3. Pfeffer L, et al. 1997. Science. 276:1418.
4. Akira S, et al. 1994. Cell. 77:63.

Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/23/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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