Purified anti-human/mouse/rat CD278 ICOS Antibody anti-CD278

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Clone: C398.4A (See other available formats)
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4
Isotype: Armenian Hamster IgG
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Product Citations: publications

C398_4A_Purified_072007 — PHA-stimulated human peripheral blood lymphocytes (3 days) stained with purified C398.4A, followed by biotinylated anti-Armenian hamster IgG and Sav-PE

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313502

500 µg

216€

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Description

ICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human, Mouse, Rat

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus, Pig

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Mouse T cell clone D10.G4.1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP, Costim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that reagents be titrated for optimal performance in the particular application.

Application Notes

The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro costimulation of T cell activation^1,3,4, and spatial biology (IBEX)^5,6. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).

Application References

Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim)
Yagi J, et al. 2003. J. Immunol. 171:783. (FC)
Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim)
Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
Sullivan KD, et al. 2021. Cell Reports. 36(7):109527. PubMed
Son YM, et al. 2020. Eur J Immunol. 50:1067. PubMed
Mann ER, et al. 2020. Sci Immunol. :5. PubMed
Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
Galbraith MD, et al. 2021. eLife. 10:00. PubMed
Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
Brenchley J, et al. 2012. Blood. 120:4172. PubMed
Gubin MM, et al. 2018. Cell. 175:1014. PubMed
Macmillan ML, et al. 2021. Blood Adv. 5:1425. PubMed
Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
Levin SD, et al. 2020. Front Immunol. 10:3086. PubMed
Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
Schwabenland M, et al. 2021. Immunity. . PubMed
Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed

RRID

AB_416326 (BioLegend Cat. No. 313502)

Antigen Details

Structure: CD28/CTLA-4, 47-57 kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781.
2. Hutloff A, et al. 1999. Nature 397:263.
3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463.
4. Coyle AJ, et al. 2000. Immunity 13:95.
Gene ID: 100048841 View all products for this Gene ID 29851 View all products for this Gene ID 64545 View all products for this Gene ID
UniProt: View information about CD278 on UniProt.org

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Other Formats

View All CD278 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IP,Costim
Biotin anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
FITC anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Alexa Fluor® 488 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,SB
Alexa Fluor® 647 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IHC-F
PerCP/Cyanine5.5 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE/Cyanine7 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Pacific Blue™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 421™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 510™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC/Cyanine7 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
PE/Dazzle™ 594 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Alexa Fluor® 700 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
APC/Fire™ 750 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 785™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 605™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Ultra-LEAF™ Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC,IP,Costim
GoInVivo™ Purified anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 711™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Brilliant Violet 650™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
TotalSeq™-B0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
TotalSeq™-C0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
TotalSeq™-A0171 anti-human/mouse/rat CD278 (ICOS)	C398.4A	PG
Brilliant Violet 750™ anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
TotalSeq™-D0171 anti-human/mouse/rat CD278 (ICOS) Antibody	C398.4A	PG
PE/Cyanine5 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC
Spark Red™ 718 anti-human/mouse/rat CD278 (ICOS)	C398.4A	FC

Certificate of Analysis

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Go To Top Version: 5 Revision Date: 08/25/2022

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