Purified anti-human CD289 (TLR9) Antibody

Pricing & Availability
Clone
S16013D (See other available formats)
Other Names
CD289, TLR9, Toll-like receptor 9
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S16013D_Purified_CD289_Antibody_1_011819.png
Human peripheral blood mononuclear cells were stained with CD304 Brilliant Violet™ 421 and CD19 APC, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) (left) or Rat IgG2a, k isotype control (right) followed by anti-rat IgG2a PE. Dot plots exclude monocytes.
  • S16013D_Purified_CD289_Antibody_1_011819.png
    Human peripheral blood mononuclear cells were stained with CD304 Brilliant Violet™ 421 and CD19 APC, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) (left) or Rat IgG2a, k isotype control (right) followed by anti-rat IgG2a PE. Dot plots exclude monocytes.
  • S16013D_Purified_CD289_Antibody_2_011819.png
    Human peripheral blood mononuclear cells were stained with CD304 Brilliant Violet™ 421 and CD19 APC, fixed, permeabilized, and then intracellularly stained with CD289 (TLR9) (clone S16013D) (left) or Rat IgG2a, k isotype control (right) followed by anti-rat IgG2a PE. Dot plots exclude monocytes.
  • S16013D_Purified_CD289_Antibody_3_020519
    Human peripheral blood was stained with CD304 Alexa Fluor® 647, CD19 Pacific Blue™, and CD289 (clone S16013D) (left) or Rat IgG2a, κ isotype control (right) followed by anti-rat IgG2a PE. Dot plots exclude monocytes and granulocytes.
Cat # Size Price Quantity Avail. Save
394802 100 µg 200€
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Description

TLR9 is a member of the toll-like receptor family that aids in recognition of pathogen associated molecular patterns (PAMPs). TLR9 recognizes unmethylated CpG sequences in DNA (ie. intracellular bacteria and DNA viruses). TLR9 signals through the MyD88 pathway leading to inflammatory cytokine production, especially production of type I interferons by pDCs.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD289 (TLR9)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC, FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

S16013D clone can be used for both surface and intracellular detection of TLR9. ICFC compatible with both the intracellular flow cytometric staining and True-Nuclear™ transcription buffer set. Does not work for WB (tested on Daudi cell line).

Antigen Details

Distribution

TLR9 is expressed by B cells and plasmacytoid dendritic cells (pDC).

Ligand/Receptor
TLR9 recognizes unmethylated CpG sequences in DNA.
Cell Type
B cells, Dendritic cells
Biology Area
Bacterial proteins and Toxins, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling, Toll Like Receptors
Antigen References
  1. Hornung V, et al. 2002. J. Immunol. 168:4531.
  2. Eaton-Bassiri A, et al. 2004. Infect. Immun. 72:7202.
  3. Krieg, A. 2007. J. Clin. Invest. 117:1184.
Gene ID
54106 View all products for this Gene ID
UniProt
View information about CD289 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
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