Purified anti-human CD255 (TWEAK) Antibody

Pricing & Availability
Clone
CARL-2 (See other available formats)
Other Names
TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
Isotype
Mouse IgM, κ
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Product Citations
publications
Carl-2_Purified_072007
Human TWEAK transfected cells stained with purified Carl-2, followed by biotinylated anti-mouse IgG and Sav-PE
  • Carl-2_Purified_072007
    Human TWEAK transfected cells stained with purified Carl-2, followed by biotinylated anti-mouse IgG and Sav-PE
  • HumanTWEAK_061604
Cat # Size Price Quantity Avail. Save
308402 100 µg 132€
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Description

TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines. The CARL-2 antibody can neutralize the bioactivity of natural or recombinant TWEAK.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
hTWEAK/2PK-3 transfectant cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
This antibody is at >85% purity.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
FC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture: The purified CARL-2 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated CARL-1 antibody (Cat. No. 308304) as the detecting antibody.
Neutralization1: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308403).

Application References
  1. Nakayama, M. 2000. J. Exp. Med. 192:1373.
RRID
AB_2206239 (BioLegend Cat. No. 308402)

Antigen Details

Structure
TNF superfamily; 17 kD (Mammalian)
Distribution

Normal tissue and tumor cells at low levels

Function
Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Bioactivity
Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Cell Sources
Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cell Targets
Macrophages, astrocytes, endothelial cells
Receptors
Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401.
3. Kaplan, M., et al. 2000. J. Immunol. 164:2897.
4. Saas, P., et al. 2000. Glia 32:102.
5. Wiley, S., et al. 2001. Immunity 15:837.

Regulation
Type II integral membrane protein readily cleaved from cell surface
Gene ID
8472 View all products for this Gene ID
UniProt
View information about CD255 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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