Purified anti-human CD23 Antibody

Pricing & Availability
Clone
EBVCS-5 (See other available formats)
Other Names
Leu-20, FcεRII, IgE Fc Receptor, BLAST-2, B6, Low affinity IgE receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
EBVCS-5_Pure_022309
Human peripheral blood lymphocytes stained with purified EBVCS-5, followed by anti-mouse IgG PE.
  • EBVCS-5_Pure_022309
    Human peripheral blood lymphocytes stained with purified EBVCS-5, followed by anti-mouse IgG PE.
Cat # Size Price Quantity Avail. Save
338502 100 µg 84€
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Description

CD23 is a 45 kD protein, also known as Leu-20, FcεRII, IgE Fc receptor, BLAST-2, B6, and low affinity IgE receptor. It is a member of the Ig family, expressed on most mature B cells, B cells in follicular mantle (but not in proliferating germinal center cells, follicular dendritic cells, monocytes, eosinophils, Langerhans cells, and a subset of T cells (10-15% of tonsillar T cells). CD23 responds to high levels of IgE by downregulating IgE secretion. In human monocytes, CD23 triggering results in release of pro-inflammatory cytokines including TNF-α, IL-1, IL-6, and GM-CSF. CD23 can be proteolytically cleaved to generate soluble CD23 fragments of various molecular weights. In chronic lymphocytic leukemia, levels of soluble CD23 in the serum can be used as a prognostic marker to identify patients at high risk for disease progression. Alternate splicing of exon 2 can also generate two cell-surface isoforms of CD23 differing by 6 amino acids in their cytoplasmic region.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References
  1. Sugden B and Metzenberg S. 1983. J. Virol. 46:800-807.
Product Citations
  1. Chevrier S 2018. Cell systems. 6:612. PubMed
Publication Library
RRID
AB_1279181 (BioLegend Cat. No. 338502)

Antigen Details

Structure
Ig family member, type II transmembrane glycoprotein, 45 kD
Distribution

B cells, follicular dendritic cells, monocytes, eosinophils, a subset of T cells, Langerhans cells

Function
Regulation of IgE synthesis in human monocytes; CD23 triggering results in release of pro-inflammatory cytokines including TNF-a, IL-1, IL-6, and GM-CSF
Ligand/Receptor
IgE, CD19/CD21/CD81
Cell Type
B cells, Dendritic cells, Eosinophils, Langerhans cells, Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Ludin C, et al. 1987. EMBO J. 6:109.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
4. Armant M, et al. 1994. J. Exp. Med. 180:1005.

Gene ID
2208 View all products for this Gene ID
UniProt
View information about CD23 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/04/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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