Purified anti-DJ-1 (PARK7) Antibody

Pricing & Availability
Clone
A16125E (See other available formats)
Other Names
Protein DJ-1, oncogene, DJ1, PARK7, Parkinson disease protein 7, Parkinsonism-Associated Deglycase, HEL-S-67p, epididymis secretory sperm binding protein Li 67p, GATD2, Protein Deglycase DJ-1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16125E_PURE_DJ-1_Antibody_1_WB_120717
Western blot of anti-DJ-1 antibody (clone A16125E). Lane 1: 20 µg of normal brain lysate; Lane 2: 20 µg of Parkinson's disease brain lysate; Lane 3: 20 ng of recombinant human DJ-1 protein that migrated as monomer (M) and dimer (D). The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • A16125E_PURE_DJ-1_Antibody_1_WB_120717
    Western blot of anti-DJ-1 antibody (clone A16125E). Lane 1: 20 µg of normal brain lysate; Lane 2: 20 µg of Parkinson's disease brain lysate; Lane 3: 20 ng of recombinant human DJ-1 protein that migrated as monomer (M) and dimer (D). The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • A16125E_PURE_DJ-1_Antibody_2_Direct-ELISA_120717
    Direct ELISA of anti-DJ-1 antibody (clone A16125E) and isotype-matched control mouse IgG2b binding to plate-immobilized recombinant human DJ-1. ELISA was performed by coating wells with 100 ng of DJ-1 protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system.
  • A16125E_PURE_DJ-1_Antibody_3_ICH-P_120717
    IHC staining of anti-DJ-1 antibody (clone A16125E) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson's disease (right panel) brain tissue. Following antigen retrieval using sodium citrate (Cat. No. 928502), the tissue was incubated with 5.0 µg/ml of the primary antibody at for one hour at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
851501 25 µg 72€
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851502 100 µg 192€
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Description

DJ-1 (PARK7) belongs to the peptidase C56 family of proteins and is expressed in almost all human cells and tissues. DJ-1 is a multi-functional protein associated with mitochondrial function, mitophagy, and male fertility. It acts as an oxidative stress sensor, redox-sensitive chaperone and protease. Therefore, the protein has an important role in cell protection against oxidative stress and cell death. DJ-1 is also associated with prostate cancer, and specific mutations of the protein are linked to autosomal recessive, early onset Parkinson’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human DJ-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA, IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For Direct ELISA, a concentration range of 0.002 - 1.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2721732 (BioLegend Cat. No. 851501)
AB_2721733 (BioLegend Cat. No. 851502)

Antigen Details

Structure
Human DJ-1 is a 189 amino acid protein with an apparent molecular weight of 20 kD. The protein consists of 7 β-strands and 7 α-helices, and exits as a dimer.
Distribution

Tissue distribution: Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in brain and placenta.
Cellular distribution: Mitochondria, nucleus, cytosol, plasma membrane, and extracellular.

Function
DJ-1 functions as an oxidative stress sensor and redox-sensitive chaperone.
Interaction
PINK1, Parkin, EFCAB6/DJBP, OTUD7B, BBS1, HIPK1, CLCF1 and MTERF.
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
Mitochondrial Markers
Antigen References

1. Saito Y. 2014. J. Clin. Biochem. Nutr. 54(3):138-44. PubMed
2. Ariga H, et al. 2013. Oxid. Med. Cell Longev. 2013:683920. PubMed
3. Bonifati V, et al. 2003. Science (5604): 256–9. PubMed

Gene ID
11315 View all products for this Gene ID
UniProt
View information about DJ-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 12/11/2017

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