- P87A11 (See other available formats)
- Other Names
- DAP6, EAP1, BING2, Death-Domain Associated Protein, Fas-Binding Protein, CENP-C Binding Protein
- Mouse IgG1, κ
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- Product Citations
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Daxx is a multifunctional protein that interacts with a wide variety of proteins. It was originally identified as a signaling protein that binds to the Fas death domain, enhances Fas-mediated apoptosis and activates the JNK pathway. Later, Daxx was found to be involved in gene regulation. It inhibits the activation of some sumoylated transcription factors such as Pax3 and Ets-1 in the nucleus. When recruited to promyelocytic leukemia protein nuclear bodies (PML-NB) through interaction with PML, its transcription repressor activity is alleviated.Product Details
- Antibody Type
- Host Species
- Purified recombinant fragment of human Daxx (aa 541-720) expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The predicted molecular weight of Daxx is 83 kD. The observed molecular weight is around 110 kD on Western blot due to post-translational hyperphosphorylation. Clone P87A11 does not cross-react with mouse (in-house tested).
AB_2632649 (BioLegend Cat. No. 691402)
- 740 amino acids with a predicted molecular weight of 83 kD. Contains a SUMO interaction motif at C-terminal region.
Mainly located in the nucleus. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress.
- Enhance Fas-mediated apoptosis. Involve in regulation of gene transcription.
- Daxx is found to have interactions with several different proteins, such as Pax3, CENP-C, DNA methyltransferase I, MCSR1, Fas, STAT3 and promyelocytic leukemia protein (PML).
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
- Antigen References
1. Yang X, et al. 1997. Cell. 89:1067-76.
2. Schreiner S, Wodrich H. 2013. J. Virol. 87:10412-22.
3. Li H, et al. 2000. Mol. Cell. Biol. 20:1784-96.
4. Takahashi Y, et al. 2004. Oncogene. 23:2819.
- Gene ID
- 1616 View all products for this Gene ID
- View information about Daxx on UniProt.org
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