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Purified anti-α-Synuclein Phospho (Ser129) Antibody (Previously Covance catalog# MMS-5091)

Pricing & Availability
Clone
P-syn/81A (See other available formats)
Regulatory Status
RUO
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease (autosomal dominant, Lewy body) 4
Previously
Covance Catalog# MMS-5091
Isotype
Mouse IgG2a, κ
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Product Citations
publications
P-synslash_81A_Purified_a-synuclein_Antibody_IHC_021715
IHC staining of purified anti-α-Synuclein Phospho (Ser129) antibody (clone P-syn/81A) on formalin-fixed paraffin-embedded diseased human brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • P-synslash_81A_Purified_a-synuclein_Antibody_IHC_021715
    IHC staining of purified anti-α-Synuclein Phospho (Ser129) antibody (clone P-syn/81A) on formalin-fixed paraffin-embedded diseased human brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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825702 25 µL 104€
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825701 100 µL 268€
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Description

α-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including  the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynapt ic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of α-synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms includes, a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy. α-synuclein is phosphorylated at low levels under normal physiological conditions whereas the majority of the protein is phosphorylated in Lewy bodies at S129.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 124 - 134 of α-synuclein, phosphorylated at Serine 129, and conjugated to KLH via a C-terminal Cysteine.
Formulation
Phosphate-buffered solution with 0.1% azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested
ICC, IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 2 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunohistochemistry (IHC-P). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemisty on frozen tissue sections (IHC-F), and immunocytochemistry.

P-syn/81A is a mAb that is specific to alpha synuclein that has been phosphorylated on serine 129.

Application References
  1. Waxman EA, Giasson BI. 2008. J. Neuropathol. Exp. Neurol. 67(5):402-16. (IHC-P, WB)
  2. Lim Y, et al. 2011. J. Neurosci. 31:10076. (IHC-P)
  3. Volpicelli-Daley LA, et al. 2014. Mol. Biol. Cell. 25:4010. (ICC) PubMed
  4. Adamowicz DH, et al. 2017. J. Neurosci. 37(7):1675-1684. (IHC-P, IHC-F) PubMed
Product Citations
  1. Dastidar SG, et al. 2020. J Neurosci. 8734:40. PubMed
  2. Schaser AJ, et al. 2019. Sci Rep. 9:10919. PubMed
  3. Yan M, et al. 2022. NPJ Parkinsons Dis. 8:1. PubMed
  4. Creed RB, et al. 2020. Neuroscience. 437:64. PubMed
  5. Doppler K, et al. 2016. Brain. 139: e5. PubMed
  6. Volpicelli-Daley L, et al. 2014. Nat Protoc. 9:2135-2146. PubMed
  7. Kim S, et al. 2019. Neuron. 103(4):627-641.e7. PubMed
  8. Adamowicz DH et al. 2017. The Journal of Neuroscience. 37(7):1675-1684 . PubMed
  9. Kim H, et al. 2022. Int J Mol Sci. 23:. PubMed
  10. Bottani E, et al. 2017. Mol Cell. 67:96. PubMed
  11. Zhang C, et al. 2021. Redox Biol. 47:102134. PubMed
  12. Lee J, et al. 2017. J Neuropathol Exp Neurol. 10.1093/jnen/nlw103. PubMed
  13. Chung CC, et al. 2021. Biomolecules. 11: . PubMed
  14. Giannoccaro MP, et al. 2021. J Parkinsons Dis. 12:585. PubMed
  15. Park HE, et al. 2019. Adv Sci (Weinh). 6:1901673. PubMed
  16. Bieri G, et al. 2019. Acta Neuropathol. 137:961. PubMed
  17. Creed RB, et al. 2022. Acta Neuropathol Commun. 10:78. PubMed
  18. Yoon YS, et al. 2022. Exp Mol Med. 54:115. PubMed
  19. Ham S, et al. 2019. Int J Mol Sci. 4.639583333. PubMed
  20. Magalhães P, et al. 2022. NPJ Parkinsons Dis. 8:93. PubMed
  21. Breid S, et al. 2016. J Virol. 90: 9182 - 9193. PubMed
  22. Zhu G, et al. 2020. Cell Rep. 108418:33. PubMed
  23. Cai W et al. 2018. EBioMedicine. 29:13-22 . PubMed
  24. Covell DJ, et al. 2017. Neuropathol Appl Neurobiol. 43:604. PubMed
  25. Kim H, et al. 2020. Int J Mol Sci. 21:00. PubMed
  26. Ryan T, et al. 2018. Nat Commun. 9:817. PubMed
  27. Yuan X, et al. 2022. Front Neurosci. 15:807988. PubMed
  28. Groveman BR et al. 2018. Acta neuropathologica communications. 6(1):7 . PubMed
  29. Sampson TR et al. 2016. Cell. 167(6):1469-1480 . PubMed
  30. Stoyka LE, et al. 2020. Neurobiol Dis. 134:104708. PubMed
  31. Stykel MG, et al. 2021. Cell Reports. 35(6):109099. PubMed
  32. Hu D, et al. 2019. Acta Neuropathol. 137:939. PubMed
RRID
AB_2734593 (BioLegend Cat. No. 825702)
AB_2564891 (BioLegend Cat. No. 825701)

Antigen Details

Structure
α-synuclein's canonical isoform consists of 140 amino acids, which consist of four 11-residue repeats containing the consensus sequence KTKEGV. α-synuclein has an apparent molecular mass of 14 kD.
Distribution

Tissue distribution: primarily nervous system, but lower expression in other tissues such as skin.
Cellular distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Function
The function of α-synuclein in the healthy brain is currently unknown.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein, Phospho-Proteins
Antigen References
  1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66. PubMed
  2. Jucker M & Walker LC. 2013. Nature. 501(7465):45. PubMed 
  3. Bartels T, et al. 2011. Nature. 477(7362):107. PubMed
  4. Devine MJ, et al. 2011. Mov. Discord. 26:2160. PubMed
Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein Phospho Ser129 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 04/25/2018

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