PerCP/Cyanine5.5 anti-mouse P2X7R Antibody

Pricing & Availability
Clone
1F11 (See other available formats)
Other Names
P2X7 receptor (P2X7R), P2RX7
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1F11_PerCPCyanine55_P2X7R_Antibody_082418
C57BL/6 splenocytes were stained with CD4 FITC and P2X7R (clone 1F11) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • 1F11_PerCPCyanine55_P2X7R_Antibody_082418
    C57BL/6 splenocytes were stained with CD4 FITC and P2X7R (clone 1F11) PerCP/Cyanine5.5 (left) or rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
148709 25 µg 112€
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148710 100 µg 256€
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Description

P2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine colon mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting1, immunohistochemistry1in vivo inhibition of intestinal inflammation and mast cell activation1.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)
RRID
AB_2728182 (BioLegend Cat. No. 148709)
AB_2728183 (BioLegend Cat. No. 148710)

Antigen Details

Structure
Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.
Distribution

Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.

Function
Ion channel activated by extracellular ATP.
Ligand/Receptor
ATP, NAD.
Cell Type
B cells, Macrophages, Mast cells, T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Surprenant A, et al. 1996. Science. 272:735.
2. Chused TM, et al. 1996. J. Immunol. 157:1371.
3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911.
4. Kawamura H, et al. 2006. J. Immunol. 176:2152.
5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908.
6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638.
7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.

Gene ID
18439 View all products for this Gene ID
UniProt
View information about P2X7R on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/24/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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