PerCP/Cyanine5.5 anti-mouse CD8b Antibody

Pricing & Availability
Clone
YTS156.7.7 (See other available formats)
Other Names
Lyt-3, Ly-3
Isotype
Rat IgG2b, κ
Ave. Rating
2 reviews
Product Citations
publications
YTS15677_PerCPCyanine55_CD8b_Antibody_083118
C57BL/6 mouse splenocytes were stained with CD3ε APC and CD8b (Clone YTS156.7.7) PerCP/Cyanine5.5 (left), or rat IgG2b, κ isotype control PerCP/Cyanine5.5 (right).
  • YTS15677_PerCPCyanine55_CD8b_Antibody_083118
    C57BL/6 mouse splenocytes were stained with CD3ε APC and CD8b (Clone YTS156.7.7) PerCP/Cyanine5.5 (left), or rat IgG2b, κ isotype control PerCP/Cyanine5.5 (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
126609 25 µg 80€
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126610 100 µg 220€
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Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations
  1. Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
  2. Acker A, et al. 2014. PLoS One. 9:110015. PubMed
  3. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  4. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  5. Gubin MM 2018. Cell. 175:1014. PubMed
  6. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  7. Sivaram N, et al. 2019. J Clin Invest. 130. PubMed
  8. Bennion BG, et al. 2019. J Virol. 93. PubMed
Publication Library
RRID
AB_961304 (BioLegend Cat. No. 126609)
AB_2260149 (BioLegend Cat. No. 126610)

Antigen Details

Structure
Ig superfamily, CD8β chain, 34 kD
Distribution

Majority of thymocytes, cytotoxic T cell subsets

Function
Co-receptor of TCR
Ligand/Receptor
MHC class I
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.

Gene ID
12526 View all products for this Gene ID
UniProt
View information about CD8b on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08/31/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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