PerCP/Cy5.5 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
3 reviews
Product Citations
publications
1A8_PerCPCy55_091809
C57BL/6 bone marrow cells stained with 1A8 PerCP/Cy5.5
  • 1A8_PerCPCy55_091809
    C57BL/6 bone marrow cells stained with 1A8 PerCP/Cy5.5
See PerCP/Cy5.5 spectral data
Cat # Size Price Quantity Avail. Save
127615 25 µg 76€
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127616 100 µg 236€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Kwon O, et al. 2014. Proc Natl Acad Sci U S A. 111:592. PubMed
  2. Lokken K, et al. 2014. PLoS Pathog. 10:1004049. PubMed
  3. Kovacic B, et al. 2014. Haematologica. 99:1006. PubMed
  4. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  5. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  6. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  7. Liu Y, et al. 2015. Sci Rep. 5:17215. PubMed
  8. Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed
  9. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  10. Gurung P, et al. 2016. Proc Natl Acad Sci U S A. 113: 4452-4457. PubMed
  11. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
  12. Moses K, et al. 2016. J Leukoc Biol. 99: 811 - 823. PubMed
  13. Chen Z, et al. 2016. Nat Commun. 7:11596. PubMed
  14. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  15. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  16. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
  17. Amber Smith, Amanda Smith 2016. Sci Rep. 6:38703. PubMed
  18. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  19. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  20. Bennett FC 2018. Neuron. 98:1170. PubMed
  21. Syed I 2018. Cell metabolism. 27:419. PubMed
  22. Cho SM 2018. Journal of neurochemistry. n/a. PubMed
  23. Cohen SB 2018. Cell host & microbe. 24:439. PubMed
  24. Roediger B 2018. Cell. 175:530. PubMed
  25. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  26. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  27. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  28. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  29. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  30. Misumi I, et al. 2019. Cell Rep. 27:1387. PubMed
  31. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  32. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
Publication Library
RRID
AB_1877272 (BioLegend Cat. No. 127615)
AB_1877271 (BioLegend Cat. No. 127616)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Go To Top Version: 3    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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