- Clone
- OX-86 (See other available formats)
- Other Names
- TNFRSF4, ACT35, OX-40
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse splenocytes were stimulated for three days with Con-A plus IL-2 and then stained with CD134 (clone OX-86) PE/Cyanine7 (filled histogram) or rat IgG1, κ PE/Cyanine7 isotype control (open histogram).
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 119415 | 25 µg | 84€ | ||||
| 119416 | 100 µg | 188€ | ||||
CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse OX-40-CD4 chimeric protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Clone OX-86 has been reported to act as an agonist and stimulate OX-40.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
-
- Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC)
- Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim)
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Product Citations
- RRID
-
AB_2566154 (BioLegend Cat. No. 119415)
AB_2566155 (BioLegend Cat. No. 119416)
Antigen Details
- Structure
- TNF receptor superfamily, 50 kD
- Distribution
-
Activated CD4+ and CD8+ T cells, activated B cells.
- Function
- Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28; thought to play a role in the pathogenesis of some autoimmune diseases
- Ligand/Receptor
- OX-40 ligand
- Cell Type
- B cells, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695.
2. Weinberg AD, et al. 1999. J. Immunol. 162:1818.
3. Akira H, et al. 1999. J. Immunol. 162:7058.
4. Pippig SD, et al. 1999. J. Immunol. 163:6520.
5. Higgins LM, et al. 1999. J. Immunol. 162:486. - Gene ID
- 22163 View all products for this Gene ID
- UniProt
- View information about CD134 on UniProt.org
Related Pages & Pathways
Pathways
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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