- 1A8 (See other available formats)
- Other Names
- Lymphocyte antigen 6 complex, locus G
- Rat IgG2a, κ
- Ave. Rating
- 5 reviews
- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.Product Details
- Antibody Type
- Host Species
- Ly-6G transfected EL-4J cell line.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.
Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
- Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
- Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
- Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
- Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
- Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
- Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
- Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
- Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
- Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
- Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
- Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
- Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
- Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
- Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
- Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
- Product Citations
AB_1877262 (BioLegend Cat. No. 127617)
AB_1877261 (BioLegend Cat. No. 127618)
- A 21-35 kD GPI-anchorded membrane protein
Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
- Cell Type
- Granulocytes, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Antigen References
Fleming TJ, et al. 1993. J. Immunol. 151:2399.
- Gene ID
- 546644 View all products for this Gene ID
- View information about Ly-6G on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.