PE/Cy5 anti-mouse CD122 (IL-2Rβ) Antibody

Pricing & Availability
Clone
TM-β1 (See other available formats)
Other Names
IL-2 Receptor β chain, IL-2Rβ
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
TM-b1_PECy5_CD122_Antibody_1_081817
C57BL/6 mouse splenocytes were stained with CD122 (clone TM-β1) PE/Cy5 and Brilliant Violet 421™ anti-mouse NK-1.1 Antibody (above) or Rat IgG2b, κ PE/Cy5 isotype control and Brilliant Violet 421™ anti-mouse NK-1.1 Antibody (below).
  • TM-b1_PECy5_CD122_Antibody_1_081817
    C57BL/6 mouse splenocytes were stained with CD122 (clone TM-β1) PE/Cy5 and Brilliant Violet 421™ anti-mouse NK-1.1 Antibody (above) or Rat IgG2b, κ PE/Cy5 isotype control and Brilliant Violet 421™ anti-mouse NK-1.1 Antibody (below).
  • TM-b1_PECy5_CD122_Antibody_2_081817
See PE/Cy5 spectral data
Cat # Size Price Quantity Avail. Save
123219 25 µg 104€
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123220 100 µg 248€
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Description

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The TM-β1 antibody does inhibit IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat T cell line expressing mouse CD122 ( IL-2Rβ)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of IL-2 binding1, and NK cell depletion2 in vivo. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for blocking of IL-2 binding in vivo and in vitro (Cat. No. 123204).

Application References
  1. Tanaka T, et al. 1991. J. Immunol. 147:2222.
  2. Tanaka T, et al. 1993. J. Exp. Med. 178:1103.
  3. Tanaka T, et al. 1992. Int. Immunol. 4:487.
RRID
AB_2715961 (BioLegend Cat. No. 123219)
AB_2715962 (BioLegend Cat. No. 123220)

Antigen Details

Structure
Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution
T and B cells, NK cells, monocytes, macrophages
Function
Critical component of IL-2 and IL-15 signaling
Ligand/Receptor
IL-2, IL-15
Cell Type
B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.

Gene ID
16185 View all products for this Gene ID
UniProt
View information about CD122 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08/21/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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