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PE anti-mouse GM-CSF Antibody

Pricing & Availability
Clone
MP1-22E9 (See other available formats)
Regulatory Status
RUO
Other Names
Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
Isotype
Rat IgG2a, κ
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Product Citations
publications
MP1-22E9_PE_030408
PMA+ionomycin-stimulated BALB/c mouse T cells surface were stained with CD4 PE/Cy7 and then intracellular stained with MP1-22E9 PE
  • MP1-22E9_PE_030408
    PMA+ionomycin-stimulated BALB/c mouse T cells surface were stained with CD4 PE/Cy7 and then intracellular stained with MP1-22E9 PE
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505405 25 µg 69€
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505406 100 µg 190€
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Description

GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Yeast-expressed, recombinant mouse GM-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Capture1,3-5: The purified MP1-22E9 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP1-31G6 antibody (Cat. No. 505502) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry8: The fluorochrome-labeled MP1-22E9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify GM-CSF -producing cells within mixed cell populations.
Neutralization2-4: The MP1-22E9 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo and in vitro neutralization (Cat. No. 505408).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections1,6,7, and immunocytochemistry8.

Application References
  1. Sander B, et al. 1993. J. Immunol. Methods 166:201.
  2. Suda T, et al. 1990. Cell. Immunol. 129:228.
  3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
  4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
  5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  6. Sander B, et al. 1991. Immunol. Rev. 119:65.
  7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Larkin J, et al. 2006. J. Immunol. 177:268.
Product Citations
  1. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  2. Amir M, et al. 2018. Cell Rep. 25:3733. PubMed
  3. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  4. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  5. Marks KE, et al. 2021. Cell Reports. 35(13):109303. PubMed
  6. Li J, et al. 2018. Immunity. 49:178. PubMed
  7. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  8. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  9. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  10. Jayaraman A, et al. 2017. Neurobiol Dis. 10.1016/j.nbd.2017.07.015. PubMed
  11. Chong WP, et al. 2020. Immunity. 53(2):384-397.e5.. PubMed
RRID
AB_315381 (BioLegend Cat. No. 505405)
AB_315382 (BioLegend Cat. No. 505406)

Antigen Details

Structure
Cytokine; 22 kD (Mammalian)
Bioactivity
Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Sources
T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells
Cell Targets
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Receptors
Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Biology Area
Cell Biology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Demetri, G., et al. 1991. Blood 78:2791.
3. Fan, D., et al. 1991. In vivo 5:571.
4. Negrin, R., et al. 1992. Adv. Pharmacol. 23:263.

Regulation
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Gene ID
12981 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All GM-CSF Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse GM-CSF MP1-22E9 ICFC
PE anti-mouse GM-CSF MP1-22E9 ICFC
Purified anti-mouse GM-CSF MP1-22E9 ELISA Capture,ELISPOT Capture,Neut,ICC,IHC-F
PerCP/Cyanine5.5 anti-mouse GM-CSF MP1-22E9 ICFC
APC anti-mouse GM-CSF MP1-22E9 ICFC
PE/Cyanine7 anti-mouse GM-CSF MP1-22E9 ICFC
Ultra-LEAF™ Purified anti-mouse GM-CSF MP1-22E9 ELISA Capture,ELISPOT Capture,Neut,ICC,IHC-F
PE/Dazzle™ 594 anti-mouse GM-CSF MP1-22E9 ICFC
APC/Fire™ 750 anti-mouse GM-CSF MP1-22E9 ICFC
Pacific Blue™ anti-mouse GM-CSF MP1-22E9 ICFC
Go To Top Version: 2    Revision Date: 07/08/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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