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PE anti-mouse B7-H4 (B7S1, B7X) Antibody

Pricing & Availability
Clone
HMH4-5G1 (See other available formats)
Regulatory Status
RUO
Other Names
B7H4, B7S1, B7-S1, B7x
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMH4-5G1_PE_070610
Mouse B7-H4 transfected 293 cells stained with HMH4-5G1 PE
  • HMH4-5G1_PE_070610
    Mouse B7-H4 transfected 293 cells stained with HMH4-5G1 PE
Compare all formats See PE spectral data
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139405 25 µg 117€
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139406 100 µg 308€
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Description

B7-H4 is a newly discovered B7 family member that negatively regulates T cell immunity. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockage of endogenous B7-H4 by specific monoclonal antibodies promotes T cell responses. A new reported function of B7-H4 is as an important negative regulator of innate immunity through growth inhibition of neutrophils. It has been reported that B7-H4 is not constitutively expressed on peripheral tissues but can be induced to express on T cells, B cells, macrophages, and dendritic cells. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance. Although B- and T-lymphocyte attenuator (BTLA) was proposed to be the receptor for B7-H4, further studies are needed to identify the inhibitory receptor of B7-H4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse B7-H4 human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed
  2. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
RRID
AB_10613286 (BioLegend Cat. No. 139405)
AB_10613105 (BioLegend Cat. No. 139406)

Antigen Details

Structure
Belongs to the B7 family, contains two extracellular Ig-like domains.
Distribution

Induced to express on T cells, B cells, macrophages, and dendritic cells. It is also expressed on some tumor cancer cells.

Function
Inhibit T cell activation, cell cycle progression, and cytokine production.
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Antigen References

1. Choi IH, et al. 2003. J. Immunol. 171:4650.
2. Prasad DV, et al. 2003. Immunity 18:863.
3. Kryczek I, et al. 2006. J. Exp. Med. 203:871.

Gene ID
242122 View all products for this Gene ID
UniProt
View information about B7-H4 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/08/2015

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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