- Clone
- B-D48 (See other available formats)
- Other Names
- PRF1, P1, PFP, HPLH2
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were surface stained with CD56 APC, followed by intracellular staining with Perforin (clone B-D48) PE (top) or mouse IgG1, κ PE isotype control (bottom). -
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 353303 | 25 tests | 108€ | ||||
| 353304 | 100 tests | 216€ | ||||
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
-
- RRID
-
AB_10915476 (BioLegend Cat. No. 353303)
AB_2616860 (BioLegend Cat. No. 353304)
Antigen Details
- Structure
- 70 kD
- Distribution
-
CTL, NK (cytoplasmic granules)
- Function
- Mediates targeted cell lysis.
- Cell Type
- T cells, NK cells, Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735. - Gene ID
- 5551 View all products for this Gene ID
- UniProt
- View information about Perforin on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.