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PE anti-human Perforin Antibody

Pricing & Availability
Clone
B-D48 (See other available formats)
Regulatory Status
RUO
Other Names
PRF1, P1, PFP, HPLH2
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
B-D48_PE_1_100211
Human peripheral blood lymphocytes were surface stained with CD56 APC, followed by intracellular staining with Perforin (clone B-D48) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • B-D48_PE_1_100211
    Human peripheral blood lymphocytes were surface stained with CD56 APC, followed by intracellular staining with Perforin (clone B-D48) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • B-D48_PE_2_100211
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Cat # Size Price Quantity Check Availability Save
353303 25 tests 132€
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353304 100 tests 264€
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Description

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed
  2. Figueroa-Romero C, et al. 2022. Front Immunol. 13:773288. PubMed
  3. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  4. Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed
  5. Buggert M, et al. 2014. J Immunol. 192:4685. PubMed
  6. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  7. Brumeanu TD, et al. 2020. bioRxiv. . PubMed
  8. Vardam-Kaur T, et al. 2021. Oncotarget. 12:2051. PubMed
  9. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  10. Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-1979-x. PubMed
  11. Collins DR, et al. 2021. Immunity. 54:2372. PubMed
  12. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  13. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  14. Patel T, et al. 2017. Cytotechnology. 10.1007/s10616-017-0106-3. PubMed
  15. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
RRID
AB_10915476 (BioLegend Cat. No. 353303)
AB_2616860 (BioLegend Cat. No. 353304)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis.
Cell Type
T cells, NK cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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