PE anti-human Jagged 2 Antibody

Pricing & Availability
Clone
MHJ2-523 (See other available formats)
Other Names
Jag2, Jagged-2
Isotype
Mouse IgG1, κ
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MHJ2-523_PE_012110
Human lymphoma cell line SR stained with MHJ2-523 PE
  • MHJ2-523_PE_012110
    Human lymphoma cell line SR stained with MHJ2-523 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
346904 100 tests 220€
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Description

The binding of Notch ligands with their receptors results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus. This translocation triggers a series of signaling process. The Notch receptors and their ligands are highly conserved signaling molecules throughout development. They regulate cell fate decision for various cell developments, including myogenesis, neurogenesis, and lymphocyte development. There is growing evidence that signals transmitted through different Notch ligands can differentially affect target cells. The Jagged-2 is one of the several Notch ligands identified. It is a single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL (Delta-Serrate-Lag-2) domain, followed by a tandem array of EGF-like repeats (ELRs) and cysteine-rich domain (CRD). Jagged 2 is expressed on lymphoma cells and some other tumor cells. Jagged-2 is reported to be essential for the regulation of spermatogenesis. Notch1/Jagged-2 signaling system plays an important role for male germ cells to differentiate, T cells and B cells to differentiate.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Jagged2-transfected CHO cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2128374 (BioLegend Cat. No. 346904)

Antigen Details

Structure
A single pass transmembrane protein consisting of a large extracellular N-terminal which includes the DSL domain, followed by a tandem array of EGF-like repeats (ELRs) and cysteine-rich domain (CRD).
Distribution

Expressed on lymphoma cells and some other tumor cells.

Function
Regulate cell fate decision for various cell developments, including myogenesis, neurogenesis, and lymphocyte development.
Ligand/Receptor
Notch molecules
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Antigen References

1. Moriyama Y et al. 2008. Int J. Immunol. 20:763
2. Ehebauer MT. et al. 2006. Biochem J 392:13
3. Shimizu K et al. 2000. Mol Cell Biology 20:18
4. Hellstrom M et al. 2007. Nature 445:776
5. Luo B et al. 1997. Mol Cell Biol. 17:6057
6. Stanley P et al. 2009. Immunol. Rev. 230(1):201

Gene ID
3714 View all products for this Gene ID
UniProt
View information about Jagged 2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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