Loading...

PE anti-human CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
29E.2A3 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1)
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
29E.2A3_PE_CD274_Antibody_FC_091013
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD274 (clone 29E.2A3) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 29E.2A3_PE_CD274_Antibody_FC_091013
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD274 (clone 29E.2A3) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
329705 25 tests 95€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
329706 100 tests 211€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human PD-L1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3 and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748). 

It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.

Clone 29E.2A3 does not work in Western blot applications7.

Application References
  1. Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
  3. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
  4. Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
  5. Haile, S et al. 2013. J. Immunol. 191:2829.
  6. RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
  7. Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.
Product Citations
  1. Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
  2. Cui J, et al. 2022. Cancer Cell Int. 22:72. PubMed
  3. Oreskovic E, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  4. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  5. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  6. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  7. Katsurahara K, et al. 2021. Cancer Sci. 112:1026. PubMed
  8. Geuijen C, et al. 2021. Nat Commun. 12:4445. PubMed
  9. Schott DS, et al. 2017. Oncotarget. 8:72755. PubMed
  10. Huang X, et al. 2021. Nat Nanotechnol. 16:214. PubMed
  11. Zhang H, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
  12. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  13. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  14. Sucker A, et al. 2017. Nat Commun. 8:15440. PubMed
  15. Liu Y, et al. 2021. Oncogene. 40:2230. PubMed
  16. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  17. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  18. Fujiwara Y, et al. 2021. Pancreas. 50:405. PubMed
  19. Yi K, et al. 2022. Front Immunol. 12:802795. PubMed
  20. Mineo M, et al. 2020. Molecular Cell. 78(6):1207-1223.e8. PubMed
  21. Guan H, et al. 2016. Sci Rep. 6:35651. PubMed
  22. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  23. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  24. Nduom E, et al. 2016. Neuro Oncology. 18: 195 - 205. PubMed
  25. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  26. Zong Z, et al. 2019. Front Immunol. 10:1643. PubMed
  27. Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed
  28. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  29. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  30. Bryson BD, et al. 2019. Nat Commun. 10:2329. PubMed
  31. Wu Y, et al. 2021. Nat Commun. 12:2346. PubMed
  32. Wang Y, et al. 2021. Front Immunol. 12:654463. PubMed
  33. El Ahanidi H, et al. 2022. Front Oncol. 11:795242. PubMed
  34. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  35. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  36. Rolfes V, et al. 2018. Oncotarget. 9:27460. PubMed
  37. Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed
  38. Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed
  39. Zhang R, et al. 2021. Front Oncol. 10:581733. PubMed
  40. Mao R, et al. 2020. Cancer Sci. 111:3174. PubMed
  41. Durand-Panteix S, et al. 2012. J Immunol. 189:181. PubMed
  42. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  43. Bouleau A, et al. 2022. J Nucl Med. 63:1259. PubMed
  44. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  45. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  46. Okita R, et al. 2021. Thorac Cancer. 12:775. PubMed
  47. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  48. Ye LL, et al. 2020. Annals of Translational Medicine. 8(24):1647. PubMed
  49. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  50. Yoshino H, et al. 2021. Curr Issues Mol Biol. 43:153. PubMed
  51. Iwasa M, et al. 2019. Oncotarget. 10:1903. PubMed
  52. Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
  53. Curnock AP, et al. 2021. JCI Insight. 6:. PubMed
  54. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  55. Kim DH, et al. 2019. Exp Mol Med. 51:94. PubMed
  56. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
  57. Zhou C, et al. 2021. Mol Ther. 29:1512. PubMed
  58. Ahn A, et al. 2021. Cancers (Basel). 13:. PubMed
  59. Tsuchiya K, et al. 2021. Oncoimmunology. 10:1962656. PubMed
  60. Wang J, et al. 2022. Clin Transl Med. 12:e718. PubMed
  61. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  62. Guo Q, et al. 2022. Blood Adv. 6:5668. PubMed
  63. Zhang Z, et al. 2022. Clin Transl Med. 12:e1072. PubMed
  64. Dan J, et al. 2016. J Immunol. 197: 983 - 993. PubMed
  65. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  66. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  67. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  68. Lei Y, et al. 2021. Clin Transl Immunology. 10:e1231. PubMed
  69. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  70. Sam J, et al. 2020. Front Oncol. 10:575737. PubMed
  71. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  72. Gilardini Montani MS, et al. 2020. Br J Cancer. 123:298. PubMed
  73. Xu H, et al. 2010. J Immunol. 185:7340. PubMed
  74. Zhou X, et al. 2022. J Nucl Med. 63:536. PubMed
  75. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  76. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  77. Darga EP, et al. 2021. PLoS One. 16:e0260124. PubMed
  78. Zhang H, et al. 2021. Adv Sci (Weinh). 8:2003404. PubMed
  79. Padet L, et al. 2014. Immunobiology. 219:687. PubMed
  80. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  81. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  82. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  83. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  84. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  85. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
  86. Zhang C, et al. 2020. Front Oncol. 0.944444444. PubMed
  87. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  88. Tsukamoto M, et al. 2019. Cancer Sci. 110:310. PubMed
RRID
AB_940366 (BioLegend Cat. No. 329705)
AB_940368 (BioLegend Cat. No. 329706)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells

Function
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.

Gene ID
29126 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD274 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC,IHC-P,Block
Biotin anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
PE anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
APC anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 421™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Ultra-LEAF™ Purified anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC,IHC,Block
PE/Cyanine7 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Purified anti-human CD274 (B7-H1, PD-L1) (Maxpar® Ready) 29E.2A3 FC,CyTOF®
Brilliant Violet 711™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 605™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
GoInVivo™ Purified anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC,IHC,Block
PE/Dazzle™ 594 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 785™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 510™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
PerCP/Cyanine5.5 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Brilliant Violet 650™ anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Alexa Fluor® 594 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 IHC-P
TotalSeq™-A0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
TotalSeq™-B0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
TotalSeq™-C0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
TotalSeq™-D0007 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 PG
PE/Fire™ 810 anti-human CD274 (B7-H1, PD-L1) Antibody 29E.2A3 FC
PE/Cyanine5 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 FC
Spark YG™ 570 anti-human CD274 (B7-H1, PD-L1) 29E.2A3 IHC-P,FC
Go To Top Version: 7    Revision Date: 11/30/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account