PE anti-Acetylated Lysine Antibody

Pricing & Availability
Clone
15G10 (See other available formats)
Other Names
Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Isotype
Mouse IgG2b, κ
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Product Citations
publications
15G10_PE_acetylated_lysine_Antibody_FC_1_013114
Human cervical carcinoma cell line, HeLa, was incubated with (top) or without (bottom) Trichostatin A (100 ng/ml, overnight), then treated with FOXP3 Fix/Perm Buffer Set. Cells were then stained with anti-acetylated lysine (clone 15G10) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 15G10_PE_acetylated_lysine_Antibody_FC_1_013114
    Human cervical carcinoma cell line, HeLa, was incubated with (top) or without (bottom) Trichostatin A (100 ng/ml, overnight), then treated with FOXP3 Fix/Perm Buffer Set. Cells were then stained with anti-acetylated lysine (clone 15G10) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).
  • 15G10_PE_acetylated_lysine_Antibody_FC_2_013114
See PE spectral data
Cat # Size Price Quantity Avail. Save
623404 100 tests 220€
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Description

Proteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Acetylated lysine residues
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Acetylated protein mixture
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed
Product Citations
  1. Luque–Martin R, et al. 2019. Front Pharmacol. 1.279166667. PubMed
RRID
AB_2563126 (BioLegend Cat. No. 623404)

Antigen Details

Structure
Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine
Function
Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Wang S, et al. 2004. Novartis Found. Symp. 259:238.
2. Legube G, et al. 2003. EMBO Rep. 4:944.
3. Chen LF, et al. 2003. J. Mol. Med. 81:549.

Gene ID
8850 View all products for this Gene ID
UniProt
View information about Acetylated Lysine on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/10/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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