- OKT4 (See other available formats)
- HCDM listed
- Other Names
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.Product Details
- Human, Cross-Reactivity: Chimpanzee, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Human peripheral T cells
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- test size: lot-specific; µg sizes: 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood.
It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).
In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.
- Application References
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
- Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
- Linder J, et al. 1987. Am. J. Pathol. 127:1.
- Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
- Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
- Product Citations
AB_571952 (BioLegend Cat. No. 317423)
AB_571953 (BioLegend Cat. No. 317424)
AB_1595438 (BioLegend Cat. No. 317429)
- Ig superfamily, type I transmembrane glycoprotein, 55 kD
T cell subset, majority of thymocytes, monocytes/macrophages
- MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
- MHC class II molecules, HIV gp120, IL-16
- Cell Type
- Macrophages, Monocytes, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
- Gene ID
- 920 View all products for this Gene ID
- View information about CD4 on UniProt.org
- I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
- TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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