Pacific Blue™ anti-human CD335 (NKp46) Antibody

Pricing & Availability
9E2 (See other available formats)
Regulatory Status
Other Names
NKp46, NCR1
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes stained with 9E2 Pacific Blue™ and CD56 (HCD56) PE
  • 9E2_Blue_050109.jpg
    Human peripheral blood lymphocytes stained with 9E2 Pacific Blue™ and CD56 (HCD56) PE
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331911 25 µg 113€
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331912 100 µg 235€
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CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
NKp46-Fc fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 9E2 has been shown to block NK activation through NKp46.6

Application References
  1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
  2. Kalberer CP, et al. 2003. Blood 102:127.
  3. Chen Y, et al. 2007. J. Immunol. 179:2766.
  4. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Achdout H. et al. 2010. J. Virol. 84:3993.
Product Citations
  1. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  2. Irina Bancos, Jon Hazeldine 2017. Eur J Endocrinol. 176(4):471-480. PubMed
  3. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  4. Bancos I, et al. 2017. Eur J Endocrinol. 10.1530/EJE-16-0969. PubMed
  5. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  6. Pembroke T, et al. 2014. Gut. 63:515. PubMed
AB_2149281 (BioLegend Cat. No. 331911)
AB_2149280 (BioLegend Cat. No. 331912)

Antigen Details

Type I membrane glycoprotein (46 kD)

Expressed on resting and activated NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.

Gene ID
9437 View all products for this Gene ID
View information about CD335 on

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD335 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD335 (NKp46) 9E2 FC
Biotin anti-human CD335 (NKp46) 9E2 FC
PE anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 647 anti-human CD335 (NKp46) 9E2 FC
Pacific Blue™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 421™ anti-human CD335 (NKp46) 9E2 FC
PerCP/Cyanine5.5 anti-human CD335 (NKp46) 9E2 FC
APC anti-human CD335 (NKp46) 9E2 FC
PE/Cyanine7 anti-human CD335 (NKp46) 9E2 FC
FITC anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 510™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 605™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 650™ anti-human CD335 (NKp46) 9E2 FC
PE/Dazzle™ 594 anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 700 anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 711™ anti-human CD335 (NKp46) 9E2 FC
APC/Fire™ 750 anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 488 anti-human CD335 (NKp46) 9E2 FC
TotalSeq™-B0101 anti-human CD335 (NKp46) 9E2 PG
TotalSeq™-C0101 anti-human CD335 (NKp46) 9E2 PG
TotalSeq™-A0101 anti-human CD335 (NKp46) 9E2 PG
Brilliant Violet 785™ anti-human CD335 (NKp46) 9E2 FC
Ultra-LEAF™ Purified anti-human CD335 (NKp46) 9E2 FC,FA
APC/Cyanine7 anti-human CD335 (NKp46) Antibody 9E2 FC
PE/Cyanine5 anti-human CD335 (NKp46) 9E2 FC
PE/Fire™ 810 anti-human CD335 (NKp46) 9E2 FC
TotalSeq™-D0101 anti-human CD335 (NKp46) 9E2 PG
PE/Cyanine7 anti-human CD335 9E2 FC
Spark Red™ 718 anti-human CD335 (NKp46) (Flexi-Fluor™) 9E2 FC
Go To Top Version: 2    Revision Date: 04/15/2014

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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