MojoSort™ Mouse Ly-6G Selection Kit

Pricing & Availability
Other Names
Ly6G
Ave. Rating
Submit a Review
Product Citations
publications
MojoSort_Mouse_Ly-6G_Selection_Kit_1_101618
A single cell suspension from C57BL/6 bone marrow was prepared for the positive selection of Ly-6G+ cells using the MojoSort™ Mouse Ly-6G Selection Kit. Cells were stained with anti-mouse Ly-6G (clone 1A8) PE and anti-mouse/human CD11b (clone M1/70) APC. Dead cells were excluded by Helix NP™ Blue. Left plot: Ly-6G+ cells after isolation. Right plot: Cells before isolation.
  • MojoSort_Mouse_Ly-6G_Selection_Kit_1_101618
    A single cell suspension from C57BL/6 bone marrow was prepared for the positive selection of Ly-6G+ cells using the MojoSort™ Mouse Ly-6G Selection Kit. Cells were stained with anti-mouse Ly-6G (clone 1A8) PE and anti-mouse/human CD11b (clone M1/70) APC. Dead cells were excluded by Helix NP™ Blue. Left plot: Ly-6G+ cells after isolation. Right plot: Cells before isolation.
Cat # Size Price Quantity Avail. Save
480123 20 tests 176€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
480124 200 tests 524€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Mouse Ly-6G+ cells are either selected or depleted by incubating the sample with a biotin conjugated anti-mouse Ly-6G antibody and magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the Ly-6G+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat. No. 480123:

  • 1 vial containing 200 µl of Biotin anti-mouse Ly-6G antibody (clone 1A8)
  • 1 vial containing 200 µl Streptavidin Nanobeads


For Cat. No. 480124:

  • 2 vials containing 1 ml each of Biotin anti-mouse Ly-6G antibody (clone 1A8)
  • 2 vials containing 1 ml each of Streptavidin Nanobeads

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Formulation
Antibody: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2.
Streptavidin Nanobeads: Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Conjugated nanobeads: Protein-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of Biotin anti-mouse Ly-6G antibody for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the positive selection or depletion of mouse Ly-6G+ cells from lymphoid tissue.

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 5X
Nanobead dilution to use in columns: 5X

Antigen Details

Biology Area
Immunology, Innate Immunity
Gene ID
NA
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 10/17/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

Login / Register
Forgot your password? Reset password?
Create an Account