Pricing & Availability
Regulatory Status
Other Names
SARS CoV-2, coronavirus, SARS-CoV-2 spike protein, COVID-19, 2019-nCoV, severe acute respiratory coronavirus 2 spike glycoprotein S1, SARS-CoV-2 S1 Subunit, 2019 novel coronavirus S1 protein
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Product Citations
LEGEND-MAX_SARS-CoV-2_Spike_S1_Human IgG_111120
  • LEGEND-MAX_SARS-CoV-2_Spike_S1_Human IgG_111120
Cat # Size Price Quantity Check Availability Save
447807 1 Pre-coated Plate 463€
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SARS-CoV-2 is an enveloped non-segmented positive-sense RNA coronavirus that infects the human respiratory-epithelial cells, and is the cause of the coronavirus disease 2019 (COVID-19). One of its structural proteins includes the spike protein (S). The S protein consists of the S1 subunit which contains a receptor binding domain (RBD) that is capable of recognizing and binding with the cell surface receptor, and playing a key role in viral entry. Assays to detect virus-specific antibodies are important to understand the prevalence of infection and the course of the immune system.

The BioLegend LEGEND MAX™ SARS-CoV-2 Spike S1 Human IgG ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with SARS-CoV-2 Spike Protein S1. This kit is specifically designed for the accurate quantitation of SARS-CoV-2 Spike S1 Human IgG from serum and plasma. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
SARS-CoV-2 Spike S1 Human IgG Pre-coated 96-well Strip Microplate
SARS-CoV-2 Spike S1 Human IgG Detection Antibody
SARS-CoV-2 Spike S1 Human IgG lyophilized Standard
Assay Buffer B
Wash Buffer (20X)
Substrate Solution D
Stop Solution
Plate Sealers

Product Details

Verified Reactivity


Product Citations
  1. Zhao J, et al. 2022. Proteomics Clin Appl. 16:e2200031. PubMed
Standard Range
0.234- 15 ng/mL.
Materials Not Included
Microplate reader able to measure absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
Deionized water
Wash bottle or automated microplate washer
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Polypropylene vials

Antigen Details

Biology Area
Adaptive Immunity, Bacterial proteins and Toxins, Immunology
Gene ID
View information about IgG on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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