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LEGEND MAX™ Human IFN-γ ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IFNG, IFG, IFI, interferon gamma, Immune interferon
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Product Citations
publications
human ifn-g_121709
  • human ifn-g_121709
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430107 1 Pre-coated Plate 328€
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Description

Human IFN-γ (Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-γ, IFN-gamma, etc.) is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on its anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. Natural IFN-γ is a dimeric protein with subunits of 143 amino acids. IFN-γ is synthesized as a precursor protein of 166 amino acids including a secretory signal sequence of 23 amino acids. The protein is glycosylated.

BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IFN-γ specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IFN-γ ELISA Kit is specifically designed for the accurate quantitation of Human IFN-γ from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human IFN-γ Pre-coated 96-well Strip Microplate
  • Human IFN-γ Detection Antibody
  • Human IFN-γ Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA
Application References
  1. Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed
  2. Johnston LR, et al. 2015. Atherosclerosis. 239:283. PubMed
Product Citations
  1. Henault J, et al. 2015. Nat Immunol. 17: 196-203. PubMed
  2. Liu T, et al. 2021. Gut. 70:1965. PubMed
  3. Saito T, et al. 2014. Biochem Biophys Res Commun. 456:799. PubMed
  4. Zhou Z, et al. 2022. Front Pharmacol. 13:870178. PubMed
  5. Zeng W, et al. 2021. Exp Ther Med. 22:1186. PubMed
  6. Weist MR, et al. 2018. J Nucl Med. 59:1531. PubMed
  7. Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed
  8. Johnston L, et al. 2015. Atherosclerosis. 239:283. PubMed
  9. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  10. Guo J, et al. 2019. Cancer Immunol Res. 1.349305556. PubMed
  11. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  12. Zeng Y, et al. 2021. Aging (Albany NY). 13:. PubMed
  13. Do P, et al. 2020. ACS Comb Sci. 533:22. PubMed
  14. Damodharan SN, et al. 2020. Cytotherapy. 22:450. PubMed
  15. Brown CE, et al. 2018. Mol Ther. 26:31. PubMed
Sensitivity
5.6 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
CD8+ and CD4+ T cells, NK cells
Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 3    Revision Date: 09/13/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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