LEGEND MAX™ Human GM-CSF ELISA Kit

Pricing & Availability
Other Names
GM-CSF Pre-coated ELISA Kit
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Product Citations
publications
human gm-csf_121709
  • human gm-csf_121709
Cat # Size Price Quantity Save
432007 1 Pre-coated Plate 280€
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Description

Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.

BioLegend’s LEGEND MAX™ Human GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human GM-CSF specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human GM-CSF ELISA Kit is specifically designed for the accurate quantitation of Human GM-CSF from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human GM-CSF Pre-coated 96-well Strip Microplate
  • Human GM-CSF Dectection Antibody
  • Human GM-CSF Standard
  • Matrix A
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Application

ELISA

Application References
  1. Samavedam UK, et al. 2014. J. Immunol. 192:559. PubMed
Product Citations
  1. Samavedam U, et al. 2014. J Immunol. 192:559. PubMed
Publication Library
Sensitivity
3.5 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
T cells, macrophages, fibroblasts, endothelial cells, mast cells
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Gene ID
1437 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

Dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The Wash buffer is the same for all the current LEGEND MAX™ kits. All the part numbers on the Wash Buffer bottles in these kits should be the same. For ELISA MAX™ Deluxe and ELISA MAX™ Standard sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for a washing of the plates before even adding any sample to it. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The washings were designed to remove these components before you start the assay. If you do not do the washings, the effect on assay performance is negligible.

Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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