LEAF™ Purified anti-human/mouse Latent TGF-β Antibody

Pricing & Availability
Clone
TW7-7H4 (See other available formats)
Other Names
Latent TGF-β1, Latent TGFβ, Latent TGFβ1, LTGF-β1, LTGF-β, LTGFβ1, LTGFβ, Latent transforming growth factor beta
Isotype
Mouse IgG1, κ
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Product Citations
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Cat # Size Price Quantity Avail. Save
361202 500 µg 256€
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Description

Latent TGF-β is secreted as a complex consisting of LAP and TGF-β1. LAP and TGF-β1 are synthesized as a single propeptide precursor of 390 amino acids with an N-terminal signal peptide of 29 amino acids, a 249 amino acids pro-region (LAP), and a 112 amino acids C-terminal region (TGF-β1).

Both LAP and TGF-β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-β1 remain non-covalently associated, forming the small latent TGF-β complex (SLC, 100 kD). The large latent TGF-β complex (LLC, 235-260 kD) contains a third component, the latent TGF-β binding protein (LTBP), which is linked to LAP by a single disulfide bond. LTBP does not confer latency but is for efficient secretion of the complex to extracellular sites.

TGF-β1 and LAP can be released (activated) by many factors, including enzymes and low or high pH. TGF-β1 and LAP have multiple functions, such as regulating proliferation and differentiation of various cell types.

Increased production and activation of latent TGF-β have been linked to immune defects associated with malignancy and autoimmune disorders, susceptibility to opportunistic infection, and to the fibrotic complications associated with chronic inflammatory conditions.

Latent TGF-β has been found to protect against glomerulonephritis. It is also a surface marker of activated regulatory T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Latent TGF-β
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.  Concentrations of 1 µg/ml and 0.5 µg/ml of the capture antibody were utilized to generate the example standard curves for human latent TGF-β and mouse latent TGF-β, respectively. It is recommended that each lot of reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture:
To measure human latent TGF-β, this antibody can be used as a capture antibody in sandwich ELISA format, and paired with the biotinylated TW4-6H10 antibody (Cat. No. 349707) as the detection antibody. Recombinant Human Latent TGF-β (ELISA Std.) (Cat. No. 593509) can be used as the protein standard.
To measure mouse latent TGF-β, this antibody can be used as a capture antibody in sandwich ELISA format, and paired with the biotinylated TW7-16B4 antibody (Cat. No. 141411) as the detection antibody. Recombinant Mouse Latent TGF-β (ELISA Std.) (Cat. No. 594509) can be used as the protein standard.

Note: For testing human latent TGF-β in serum, plasma or supernatant, LEGEND MAX™ Human Latent TGF-β ELISA Kit (Cat. No. 432907 & 432908) is specially developed and recommended. For testing mouse latent TGF-β, LEGEND MAX™ Mouse Latent TGF-β ELISA Kit (Cat. No. 433007 & 433008) is recommended.

RRID
AB_2563029 (BioLegend Cat. No. 361202)

Antigen Details

Structure
Homodimer
Distribution

Latent TGF-β is secreted by numerous cells, and activated Treg cells.

Function
Latent TGF-β is the inactive form of TGF-β1 and LAP.
Ligand/Receptor
Latent TGF-β binding proteins (LTBPs)
Cell Type
Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Lu M, et al. 2002. J. Cell Sci. 115:4641.
2. Khalil N. 1999. Microbes. Infect. 1:1255.
3. Probst-Kepper M and Buer J. 2010. Biol. Direct 5:8.
4. Ali N, et al. 2008. PLoS One 3:e1914.

Gene ID
7040 View all products for this Gene ID
UniProt
View information about Latent TGF-beta on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02/14/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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