LEAF™ Purified anti-human CD221 (IGF-1R) Antibody

Pricing & Availability
1H7/CD221 (See other available formats)
Other Names
Insulin-like growth factor 1 receptor (IGF1R)
Mouse IgG1, κ
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Human peripheral blood lymphocytes were stained with purified CD221 (clone 1H7/CD221) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by biotinylated anti-mouse IgG and Sav-PE.
  • 1H7slashCD221_Pure_042211
    Human peripheral blood lymphocytes were stained with purified CD221 (clone 1H7/CD221) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by biotinylated anti-mouse IgG and Sav-PE.
Cat # Size Price Quantity Avail. Save
351804 500 µg 300€
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This product is scheduled for discontinuation as a part of our ongoing LEAF™ to Ultra-LEAF™ antibody transition. The equivalent Ultra-LEAF™ product may be available at comparable or significantly lower price. Please use the availability checker to determine current availability, or contact technical service for further inquiries.

CD221, also known as insulin-like growth factor 1 receptor (IGF1R), is a 135 kD transmembrane receptor. It is widely expressed on lymphoid and myeloid cells as well as non-haematopoietic cells. CD221 is a tyrosine kinase receptor and the ligands for CD221 are insulin-like growth factor 1 and 2 (IGF-1 and IGF-2), the polypeptide hormones similar in molecular structure to insulin. IGF-1/2 binding activates downstream PI3K and MAPK pathways and plays an important role in cell growth. Recent studies reveal its function of recruiting immune cells during tissue repair.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
IGF-1 receptor purified from human placentas
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

FC - Quality tested
Block, IHC, IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has been published as 1H7. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining of frozen tissue sections2, and blocking functions2-5.

This product is offered for sale and sold pursuant to a license to seller by City of Hope and may be used only as a research reagent.  Use for any other purpose is expressly not authorized.

Application References
  1. Li SL, et al. 1993. Biochem. Bioph. Res. Co. 196:92. (WB IP)
  2. Li SL, et al. 2000. Cancer Immunol. Immun. 49:243. (IHC Block)
  3. Ye JJ, et al. 2003. Horm. Metab. Res. 35:836. (Block)
  4. Kusada Y, et al. 2008. J. Biochem. 143:9. (Block)
  5. Ohtani M, et al. 2009. Biosci. Trends. 3:131. (Block)
AB_10900245 (BioLegend Cat. No. 351804)

Antigen Details

IGF-1R contains 2 α subunits and 2 β subunits, 135 kD

Haematopoietic cells and non-haematopoietic cells

Cell growth and immune cells recruitment
IGF-1, IGF-2
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Baudler S, et al. 2005. J. Immunol. 174:5516.
2. Sharp LL, et al. 2005. Nat. Immunol. 6:73.

Gene ID
3480 View all products for this Gene ID
View information about CD221 on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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