ELISA MAX™ Deluxe Set Human IL-1β

Pricing & Availability
Regulatory Status
Other Names
IL-1b Elisa Kit
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Product Citations
  • Human_IL-1beta_012011
Cat # Size Price Quantity Check Availability Save
437004 5 plates 236,00€
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437015 10 Plates 400,00€
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437016 20 Plates 640,00€
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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

ELISA Max™ Set Deluxe includes pre-titrated capture antibody, pre-titrated biotinylated detection antibody, pre-titrated Avidin-HRP, conveniently-sized assay calibration standards, easy-to-follow instructions, as well as, assay diluent, coating buffer,  and TMB substrate. Complete and ready to use!

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human IL-1β ELISA MAX™ Capture Antibody (200X)
  • Human IL-1β ELISA MAX™ Detection Antibody (200X)
  • Human IL-1β Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
  • Assay Buffer D

Product Details



As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

BioLegend's mouse and human IL-1ß ELISA kits use antibodies raised against the mature form of IL-1ß. However, because pro-IL-1ß contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1ß ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1ß.

Product Citations
  1. Niemiec M, et al. 2017. BMC Genomics. 10.1186/s12864-017-4097-4. PubMed
  2. Huang HY, et al. 2018. Int Endod J. 9:1425. PubMed
  3. Orzalli MH, et al. 2018. Mol Cell. 71:825. PubMed
  4. Aarreberg LD, et al. 2019. Mol Cell. 74:801. PubMed
  5. Wurster S, et al. 2017. Virulence. 8:1708. PubMed
  6. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  7. Fuchs T, et al. 2016. Immunol Lett. 173:21-25. PubMed
  8. Ireland D, et al. 2016. Innate Immunity. 10.1177/1753425916672313. PubMed
  9. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  10. Iannucci J, et al. 2020. Cell Mol Neurobiol. . PubMed
  11. Wang X, et al. 2021. Front Pharmacol. 12:673818. PubMed
  12. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
  13. Hoang QTM, et al. 2021. Front Med (Lausanne). 8:676058. PubMed
0.5 pg/mL
Standard Range
2.0-125 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH 7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (2N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Plate Shaker
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
3553 View all products for this Gene ID
View information about IL-1beta on UniProt.org

Related FAQs

Do your IL-1β ELISA kits detect pro-IL-1β or the mature form?

The antibodies in our IL-1β kits were generated against the mature form of the protein. However, because the pro-form of the protein contains the mature form, the antibodies should detect both forms.

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 7    Revision Date: 11/16/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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