Go-ChIP-Grade™ Purified anti-IRF3 Antibody

Pricing & Availability
Clone
12A4A35 (See other available formats)
Other Names
Interferon regulatory factor-3 (IRF-3)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
12A4A35_GoChIP_IRF3_Antibody_120717
Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with LPS with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF3 (Clone 12A4A35), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IFNB gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 12A4A35_GoChIP_IRF3_Antibody_120717
    Chromatin Immunoprecipitation (ChIP) was performed using Go-ChIP-Grade™ Protein G Enzymatic Kit by loading 3 µg of cross-linked chromatin samples from THP-1 cells treated with LPS with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-IRF3 (Clone 12A4A35), or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IFNB gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
  • 12A4A35_Purified_IRF3_Antibody_112817
    Total lysates (15 µg protein) from Daudi (lane 1) and A20 (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF3 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH and chemiluminescence detection. GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: Molecular weight ladder.
  • 12A4A35_Purified_IRF3_Antibody_IF_062013
    Jurkat cells were stained with purified anti-IRF3 (12A4A35) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
Cat # Size Price Quantity Avail. Save
655703 25 µg 60€
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655704 100 µg 188€
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Description

IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial IRF-3 recombinant protein (200-427 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IF, WB - Validated 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16  immunoprecipitations, respectively, at the recommended dilution.

RRID
AB_2721589 (BioLegend Cat. No. 655703)
AB_2721590 (BioLegend Cat. No. 655704)

Antigen Details

Structure
427 amino acids, predicted molecular weight of 47 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding.
Distribution

Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.

Function
IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.
Interaction
CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
2. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325.
3. Molle C, et al. 2010. J. Immunol. 184:1784.

Gene ID
3661 View all products for this Gene ID
UniProt
View information about IRF3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 12/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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