FITC Rat IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK4530 (See other available formats)
Isotype
Rat IgG2b, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
400633 25 tests 24€
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400605 50 µg 28€
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400634 100 tests 76€
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400606 200 µg 100€
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Description

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
test sizes: lot-specific; µg sizes: 0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this rat IgG2b, ? isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin <  0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.

Application References
  1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
  2. Zeiser R, et al. 2007. Blood 109:2225.
  3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
  4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
  5. Yi H, et al. 2009. Blood 113:5819. PubMed
  6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
  7. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
  8. Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations
  1. Wang F, et al. 2018. Oncogene. 37:286. PubMed
  2. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  3. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  4. López MJ, et al. 2018. Invest Ophthalmol Vis Sci. 59:5671. PubMed
  5. Grisanti LA, et al. 2019. JCI Insight. 5. PubMed
  6. Hao T, et al. 2019. Nat Commun. 10:3665. PubMed
  7. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  8. Ohgomori T et al. 2019. J Comp Neurol. 528(1):14-31 . PubMed
  9. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  10. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  11. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  12. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  13. Gupta P, et al. 2016. PLoS One. 11: 0154725. PubMed

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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