FITC anti-mouse Lineage Cocktail with Isotype Ctrl

Pricing & Availability
Clone
145-2C11, RB6-8C5, M1/70, RA3-6B2, Ter-119 (See other available formats)
Other Names
Lin-1
Isotype
Armenian Hamster IgG, Rat IgG2b, Rat IgG2a
Ave. Rating
1 reviews
Product Citations
publications
A_FITC_Lineage_042909.jpg
C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 (clone RAM34) Alexa Fluor® 647
  • A_FITC_Lineage_042909.jpg
    C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 (clone RAM34) Alexa Fluor® 647
  • B_FITC_Lineage_042909.jpg
    C57BL/6 bone marrow cells stained with FITC isotype control cocktail and CD34 (clone RAM34) Alexa Fluor® 647
Cat # Size Price Quantity Avail. Save
133301 25 tests 260€
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133302 100 tests 440€
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Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3e, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

 

Materials provided:

  1. FITC anti-mCD3/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 or 100 tests
  2. FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 or 100 tests

Materials not provided:

Cell Staining Buffer (Cat. No. 420201)

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Formulation
This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation
The antibodies are purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution are free of unconjugated FITC.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solutions should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application References
  1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
  2. Okada S, et al. 1992. Blood180 (12):3044-3050
  3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
  4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
  5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
  6. Chen G, et al. 2014. Cancer Immunol Res. 2:949. PubMed
Product Citations
  1. Chen G, et al. 2014. Cancer Immunol Res. 2:949. PubMed
  2. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  3. Liu X, et al. 2015. Nat Commun. 6: 8825. PubMed
  4. Yu Q, et al. 2016. Cell Res. 10.1038/cr.2016.85. PubMed
  5. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  6. Liu J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.010. PubMed
  7. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  8. Guo XJ 2018. Immunity. 49:531. PubMed
  9. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  10. Capitano ML, et al. 2019. J Clin Invest. 130:2555. PubMed
  11. Cai Q, et al. 2018. Blood Cells Mol Dis. 71:23. PubMed
  12. Xue Y, et al. 2017. Dev Cell. 42:349. PubMed
Publication Library

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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