FITC anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
6 reviews
Product Citations
publications
IM7
C57BL/6 splenocytes stained with IM7 FITC
  • IM7
    C57BL/6 splenocytes stained with IM7 FITC
  • IM7_FITC_060710
    Human peripheral blood lymphocytes stained with IM7 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
103021 25 tests 28€
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103005 50 µg 32€
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103022 100 tests 76€
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103006 500 µg 76€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
test size: lot-specific; µg size: 0.5 mg/ml
Storage & Handling
The CD44 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been validated for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  2. Kamijo S, et al. 2013. J Immunol. 190:4489. PubMed
  3. Murai T, et al. 2013. J Cell Sci. 126:3284. PubMed
  4. Kmieciak M, et al. 2011. Breast Cancer Res Treat. 126:385. PubMed
  5. Byrne K, et al. 2014. J Immunol. 192:1433. PubMed
  6. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  7. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  8. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  9. Sim S, et al. 2016. PLoS One. 11: 0152485. PubMed
  10. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  11. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  12. Liu Z, et al. 2016. Sci Rep. 6:34416. PubMed
  13. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  14. Hsieh WC, et al. 2018. Nat Commun. 9:463. PubMed
  15. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  16. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  17. Graham JB, et al. 2017. Curr Protoc Mouse Biol. 7:221. PubMed
  18. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  19. Shah D, et al. 2018. Clin Cancer Res. 24:4566. PubMed
  20. Wang S, et al. 2019. Int J Oncol. 54:966. PubMed
  21. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  22. Matossian MD, et al. 2019. BMC Cancer. 19:205. PubMed
  23. Ahmed MF, et al. 2019. Exp Ther Med. 17:4154. PubMed
  24. Yang BH, et al. 2019. Cell Rep. 27:3629. PubMed
  25. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  26. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  27. Wang D et al. 2018. Immunity. 48(4):659-674 . PubMed
  28. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  29. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  30. Hao J et al. 2018. Cell metabolism. 28(5):689-705 . PubMed
  31. Bagheri V et al. 2018. Journal of cellular physiology. 233(10):7036-7046 . PubMed
  32. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  33. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  34. Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
  35. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  36. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  37. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  38. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  39. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  40. Wan X, et al. 2018. Nature. 560:107. PubMed
  41. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  42. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  43. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
RRID
AB_493684 (BioLegend Cat. No. 103021)
AB_312956 (BioLegend Cat. No. 103005)
AB_493685 (BioLegend Cat. No. 103022)
AB_312957 (BioLegend Cat. No. 103006)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/11/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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