FITC anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
Ave. Rating
Submit a Review
Product Citations
publications
G10F5
Human peripheral whole blood granulocytes stained with G10F5 FITC
  • G10F5
    Human peripheral whole blood granulocytes stained with G10F5 FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
305103 25 tests 68,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
305104 100 tests 156,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen and formalin-fixed paraffin-embedded tissue sections.

Application References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
Product Citations
  1. Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed
  2. Ackermann M, et al. 2018. Nat Commun. 9:5088. PubMed
  3. Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
  4. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
  5. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  6. Bonegio RG, et al. 2019. J Immunol. 202:675. PubMed
  7. Stojkov D, et al. 2017. J Cell Biol. 216:4073. PubMed
  8. Franco LM, et al. 2019. J Exp Med. 216:384. PubMed
  9. Gomez I, et al. 2020. Nat Commun. 11:214. PubMed
  10. Simon SCS, et al. 2020. Oncoimmunology. 9:1727116. PubMed
  11. Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed
  12. Nijnik A, et al. 2010. J Immunol. 184:2539. PubMed
  13. Little M, et al. 2012. PLoS One. 7:e28626.. PubMed
  14. Pliyev B, et al. 2011. Cell Immunol. 271:214. PubMed
  15. Heim C, et al. 2015. J Immunol. 194:3861. PubMed
  16. Mueller-Leisse J, et al. 2015. Med Mycol. 53: 622-629. PubMed
  17. SenGupta S, et al. 2016. J Leukoc Biol. 100: 1047 - 1059. PubMed
  18. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  19. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  20. Rodriguez-Meira A, et al. 2020. STAR Protoc. 1:100125. PubMed
  21. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  22. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  23. Pulsifer AR, et al. 2020. Infect Immun. 88:00:00. PubMed
  24. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  25. Margaroli C, et al. 2021. Cell Reports Medicine. 2(4):100239. PubMed
  26. Reif T, et al. 2021. Cell Reports Medicine. 2(6):100317. PubMed
RRID
AB_314495 (BioLegend Cat. No. 305103)
AB_314496 (BioLegend Cat. No. 305104)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution
Granulocytes
Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org

Related FAQs

There are no FAQs for this product.

Customers Also Purchased

Go To Top Version: 2    Revision Date: 01/05/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account