Cyanine3 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
SAv-Cy3
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Product Citations
publications
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405215 100 µg 95€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Cy3 is useful for detecting biotinylated antibodies. The excitation of Cy3 by 550 nm laser light induces a light emission maximum of 570 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with Cyanine3 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-Cy3 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F, IHC-P

Recommended Usage

It is recommended that the reagent be titrated for optimal performance for each application. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Cyanine3 conjugation.

Application Notes

The average molecular weight of Streptavidin-Cy3 is 60 kD and Streptavidin alone is 52 kD.

Additional Product Notes
BioLegend is in the process of converting the name Cy3 to Cyanine3. The dye molecule remains the same, so you should expect the same quality and performance from our Cyanine3 products. Please contact Technical Service if you have any questions.
Application References
  1. Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed
  2. Thoma CR, et al. 2013. J Biomol Screen. 18:1330. PubMed
  3. Sunshine J, et al. 2014. J. Virol. 88:1354. PubMed
  4. Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed
  5. Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed
Product Citations
  1. Kai D, et al. 2014. Acta Biomater. 10:2727. PubMed
  2. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  3. Atreya I, et al. 2016. J Crohns Colitis. 10: 1132 - 1143. PubMed
  4. Kelm W 2013. J Biomol Screen. 18:1330. PubMed
  5. Heidler J, et al. 2013. Dis Model Mech. 6:1378. PubMed
  6. Silginer M, et al. 2014. Cell Death Dis. 5:1012. PubMed
  7. Sunshine J, et al. 2014. J Virol. 88:1354. PubMed
  8. Fukuyama T, et al. 2015. J Pharmacol Exp Ther. 354: 394 - 405. PubMed
  9. Sharma N, et al. 2015. J Immunol. 195: 5227 - 5236. PubMed
  10. Okamura Y, et al. 2017. Allergol Int. 10.1016/j.alit.2017.03.002. PubMed

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Go To Top Version: 5    Revision Date: 12/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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